中国少数民族全基因组DNA高通量提取及其样本库建立

doi:10.3969/j.issn.1000-1565.2016.02.010

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (2): 169-174.DOI: 10.3969/j.issn.1000-1565.2016.02.010

中国少数民族全基因组DNA高通量提取及其样本库建立

张一鸣,王俊丽,黄婧婧,张荣荣,彭耀

收稿日期:2015-06-25 出版日期:2016-03-25 发布日期:2016-03-25
通讯作者: 王俊丽(1964-),女,河北新乐人,中央民族大学教授,博士生导师,主要从事植物细胞工程及资源利用研究.E-mail:wangjunli1698@163.com
作者简介:张一鸣(1986-),男,辽宁本溪人,中央民族大学在读博士研究生. E-mail:61705358@qq.com
基金资助:
国家重大科学仪器设备开发专项(2012YQ03026108);高等学校学科创新引智计划资助项目(B08044);一流大学一流学科建设项目(YLDX01013)

High-throughput extraction and establishment of sample bank of the genome-wide DNA of different Chinese ethnic minorities

ZHANG Yiming,WANG Junli,HUANG Jingjing,ZHANG Rongrong,PENG Yao

College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China

Received:2015-06-25 Online:2016-03-25 Published:2016-03-25

摘要/Abstract

摘要： 为保存中国不同民族基因组遗传资源,建立中国少数民族全基因组DNA样本库.按照临床采血标准,遵守知情同意原则,采集不同民族志愿者外周血2 mL,采用全自动核酸提取设备高通量提取DNA,经质量控制后编号入库,建立了包含545例样本的中国少数民族全基因组DNA样本库.本研究将为研究人类起源、环境与遗传背景相互作用提供物质基础,为探索疾病病因、诊断技术和治疗策略提供资源保障.

关键词: 少数民族, 全基因组, DNA样本库

Abstract: To preserve genetic resources of different Chinese ethnic groups’ genomes and establish the sample bank of the genome-wide DNA,2 mL peripheral blood was collected from each volunteer of different ethnic minorities according to the clinical blood collection standards and complying with the principle of informed consent.Automatic nucleic acid extraction machine was used to conduct high-throughput extraction of all blood samples,and the samples were numbered and put in the bank after passing quality control.The established sample bank of the genome-wide DNA includes 545 samples from ethnic minorities.The bank will provide material basis for the research of anthropogenesis and interaction of environment and genetic background;it will also serve as a resource for disease treatment and diagnostics.

Key words: ethnic minorities, genome-wide, DNA sample bank

中图分类号:

张一鸣,王俊丽,黄婧婧,张荣荣,彭耀. 中国少数民族全基因组DNA高通量提取及其样本库建立[J]. 河北大学学报(自然科学版), 2016, 36(2): 169-174.

ZHANG Yiming,WANG Junli,HUANG Jingjing,ZHANG Rongrong,PENG Yao. High-throughput extraction and establishment of sample bank of the genome-wide DNA of different Chinese ethnic minorities[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(2): 169-174.

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中国少数民族全基因组DNA高通量提取及其样本库建立

High-throughput extraction and establishment of sample bank of the genome-wide DNA of different Chinese ethnic minorities

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