不同浓度尿酸对体外红细胞流变学指标的影响

doi:10.3969/j.issn.1000-1565.2016.02.013

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (2): 184-188.DOI: 10.3969/j.issn.1000-1565.2016.02.013

不同浓度尿酸对体外红细胞流变学指标的影响

李葵花¹,李璐²,严鹏¹,佟晓波¹,谢利德¹

收稿日期:2015-04-18 出版日期:2016-03-25 发布日期:2016-03-25
作者简介:李葵花(1978—),女,河北保定人,承德医学院副教授,主要从事血液流变学研究. E-mail:148117940@qq.com
基金资助:
河北省高等学校科学技术研究项目(QN20131033);承德医学院自然科学一般项目(201313);承德医学院大学生科研立项(201439)

Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro

LI Kuihua¹,LI Lu²,YAN Peng¹,TONG Xiaobo¹,XIE Lide¹

1.Department of Biomedical Engineering, Chengde Medical College, Chengde 067000, China; 2.Department of Anesthesiology, Affiliated Hospital of Hebei University, Baoding 071000, China

Received:2015-04-18 Online:2016-03-25 Published:2016-03-25

摘要/Abstract

摘要： 探讨了不同浓度尿酸水平对体外红细胞流变学指标的影响.300、600、1 200 μmol/L的尿酸对体外红细胞作用2 h后,测量红细胞的流变学指标,并应用SDS-PAGE对红细胞膜蛋白成分进行分析.随尿酸浓度的增加,体外红细胞的积分变形指数、松弛指数降低,红细胞的破碎率升高.与对照组相比,红细胞锚蛋白及带3蛋白含量减少,1 200 μmol/L尿酸组的相关指标具有明显差别.提示高浓度尿酸可对红细胞膜蛋白产生明显的破坏作用,是引起红细胞流变学特性改变的独立因素.

关键词: 尿酸, 红细胞, 血液流变学, 膜蛋白

Abstract: The effects of different concentrations of uric acid on hemorheology were investigated in vitro.The erythrocytes were suspended in 300,600,1 200 μmol/L uric acid for 2 h and the hemorheological parameters were measured.The membrane protein composition was also analyzed by SDS-PAGE.We found that the integrated deformation index and relax index were reduced in a dose-dependent manner.The hemolysis rate increased.As compared with the control group,the content of ankyrin and band 3 proteins showed a decrease trend.These results were statistically significant in the 1 200 μmol/L group.The results suggest that high concentrations of uric acid can have significant damaging effects on erythrocyte membrane proteins,which is an independent factor affecting the hemorheological properties of erythrocytes.

Key words: uric acid, erythrocyte, hemorheology, membrane proteins

中图分类号:

李葵花,李璐,严鹏,佟晓波,谢利德. 不同浓度尿酸对体外红细胞流变学指标的影响[J]. 河北大学学报(自然科学版), 2016, 36(2): 184-188.

LI Kuihua,LI Lu,YAN Peng,TONG Xiaobo,XIE Lide. Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(2): 184-188.

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不同浓度尿酸对体外红细胞流变学指标的影响

Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro

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