9种拟步甲16S rDNA部分序列及其亲缘关系

doi:10.3969/j.issn.1000-1565.2004.04.015

摘要/Abstract

摘要： 测定了9种拟步甲的16S rDNA部分基因序列,并与GenBank中的1种步甲的基因序列作同源性比较,计算其核苷酸使用频率并构建了分子系统树.在获得的435 bp的序列中,A+T约占74.4%,颠换(transversion)取代的速率大于转换(transition)取代的速率,其中277个核苷酸位点存在变异.结果表明:属内种间的碱基序列差异范围为3.4%～6.2%;族内属间为9.4%～11.0%;科内族间为10.8%～17.7%;科与科间的差异达到46.7%～50.3%.分子系统树表明:拟步甲科为一单系群,其中琵甲族较为进化,漠甲族与漠王族相对原始;琵甲族与土甲族的亲缘关系较近;漠甲族、漠王族与鳖甲族的亲缘关系较近.本结果与传统的分类观点相吻合.

关键词: 鞘翅目, 拟步甲, mt DNA, 16S rDNA, 分子系统学

中图分类号:

Q969.226

刘晓丽,任国栋. 9种拟步甲16S rDNA部分序列及其亲缘关系[J]. 河北大学学报(自然科学版), 2004, 24(4): 399-405.

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