中药毛细管电泳谱图的可视化比照

doi:10.3969/j.issn.1000-1565.2010.02.010

河北大学学报(自然科学版) ›› 2010, Vol. 30 ›› Issue (2): 152-157.DOI: 10.3969/j.issn.1000-1565.2010.02.010

中药毛细管电泳谱图的可视化比照

张红医,王艳霞,景聪,陈辉

河北大学化学与环境科学学院,河北,保定,071002

出版日期:2010-03-25 发布日期:2010-03-25
基金资助:
国家自然科学基金面上项目，河北省自然科学基金

Improvement of Visualization Comparison for Traditional Chinese Medicine Electropherogram by Transformation Time x-scale

ZHANG Hong-yi,WANG Yan-xia,JING Cong,CHEN Hui

Online:2010-03-25 Published:2010-03-25

摘要/Abstract

摘要： 提出了一种新的谱图记录模式,以迁移时间t与分离电压V的乘积的负倒数(-(1/t·V))来代替原来的迁移时间t作为新谱图的横坐标,并在新坐标体系中选择合适的参照物谱峰,对变换后的电泳谱图实施平移操作.选择中药赤芍为分析对象考察了这种新变换在区带电泳和胶束电动色谱条件下对重现性的影响.实验表明对于不同电压下的电泳谱图,由处理前的迁移时间相对标准偏差(RSD)33%,降为处理后的(-(1/t·V))RSD 1.5%;对于平行实验间电泳谱图,由处理前的迁移时间RSD介于0.90%～1.39%, 降为处理后的(-(1/t·V))RSD介于0.17%～0.70%.这些结果表明,本文提出的谱图变换方式可显著提高谱图可视化比较的能力.

关键词: 毛细管电泳谱图, 记录模式, 可视化

Abstract: A new electropherogram mode was proposed by transforming time x-scale into minus reciprocal of the product of migration time and applied voltage. After a proper peak in the new electropherogram was assigned as the reference, each electropherogram was then moved along x-scale at a proper distance. The positive effect of new electropherogram on visual comparison was demonstrated by analyzing Radix paeoniae rubrain in capillary zone electrophoresis and mecellar electrokinetic chromatography. The relative standard deviation (RSD) in migration time was around 33% for each chosen peak when applied voltage varied from 15 kV to 30 kV, while the RSDs were dropped into the range from 0.17% to 0.70%. All results indicated that the proposed transformation method can significantly improve the visualization comparison of the electropherograms obtained at different voltages, in different sample matrices.

Key words: electropherogram, recording mode, visualization

中图分类号:

O657.8

张红医,王艳霞,景聪,陈辉. 中药毛细管电泳谱图的可视化比照[J]. 河北大学学报(自然科学版), 2010, 30(2): 152-157.

ZHANG Hong-yi,WANG Yan-xia,JING Cong,CHEN Hui. Improvement of Visualization Comparison for Traditional Chinese Medicine Electropherogram by Transformation Time x-scale[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(2): 152-157.

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中药毛细管电泳谱图的可视化比照

Improvement of Visualization Comparison for Traditional Chinese Medicine Electropherogram by Transformation Time x-scale

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