表面连接与核-壳型键合FITC荧光纳米硅球的制备及性能比较

doi:10.3969/j.issn.1000-1565.2017.03.005

摘要/Abstract

摘要： 以异硫氰酸荧光素(FITC)为荧光分子,二氧化硅为生物相容性载体制备荧光纳米硅球.为增强硅球的稳定性,引入γ-氨丙基三乙氧基硅烷(APTES)做桥梁分子,由于FITC、SiO₂与APTES连接顺序的非单一性,分别制备了表面连接型和核-壳型键合FITC的荧光硅球,并对其形貌和光漂白性进行了比较.实验结果表明,核-壳型和表面连接型荧光硅球形貌相似粒径接近,但表面连接型光漂白性严重.进而,对核-壳型中典型的包硅方法——Stöber法和反相微乳液法进行比较,结果表明,反相微乳液法制备的核-壳型键合FITC的荧光硅球荧光信号更强、粒径更小且分散性良好,同时在7 h内荧光染料泄漏程度小于8%,30 min内荧光强度仅降低2.2%,具备低染料泄露、光稳定性好等优点.

关键词: 异硫氰酸荧光素(FITC), 荧光纳米硅球, 光漂白, 染料泄漏, 光稳定

Abstract: Silica fluorescent nanoparticles were prepared with fluorescein isothiocyanate(FITC)as the fluorescent molecule and silica as the biocompatible carrier.To enhance the stability of the microspheres,the bridge molecule(γ-aminopropyl triethoxysilane,APTES)was introduced.Owing to the non-unity of the connection sequences of FITC,SiO₂ with APTES,two types(surface connection type and core-shell type)of FITC bonded fluorescent silica nanospheres were prepared and compared in their morphologies and photobleachings.The results show that both types had similar morphology,but the surface connection type was optically bleached seriously.Furthermore,two typical methods of packaging silicon(Stöber and reverse microemulsion)were compared.Core-shell FITC bonded fluorescent silica nanospheres preparaed by reverse microemulsion method showed stronger fluorescence signal,and had smaller particle size and better dispersibility.In addition the value of fluorescent dye leak was less than 8% within 7 h in the dye leakage experiment and the fluorescence intensity decreased only 2.2% within 30 min in light stability experiment,which showed the low dye leakage degree and good light stability properties.-

Key words: fluorescein isothiocyanate(FITC), fluorescent silica nanospheres, photobleaching, dye leakage, light stability

中图分类号:

O613.7

蔡少凡,金秋,魏海英,吕运开. 表面连接与核-壳型键合FITC荧光纳米硅球的制备及性能比较[J]. 河北大学学报(自然科学版), 2017, 37(3): 248-253.

CAI Shaofan,JIN Qiu,WEI Haiying,LÜ Yunkai. Preparation and characterization comparison of FITC bonded fluorescent silica nanospheres with surface connection type and core-shell type[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(3): 248-253.

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