关键词: 紫叶李, 总多酚, 响应面法, 提取工艺, 抗炎活性

Abstract: This work aims to optimize the extraction technology of total polyphenols(PTP)from Prunus cerasifera and study its anti-inflammatory activity.Based on the results of the single-factor experiment,using extraction rate of total polyphenols as an index,volume fraction of ethanol,extraction time,extraction temperature and solid-liquid ratio as factors,the Box-Behnken experimental design and the response surface methodology were applied to optimize the extraction technology of PTP.The anti-inflammatory activities of PTP were studied via ear swelling induced by xylene and toe swelling induced by albumen.The results showed that the optimum extraction conditions of PTP were as follows: volume fraction of ethanol 69%,extraction time 1.5 h,extraction temperature 81 ℃,solid-liquid ratio at 1∶31(g∶mL).Under these conditions,the yield of PTP was 55.3 mg/g.PTP could effectively inhibit the inflammation of ear swelling of mouse and toe swelling of rats.The results suggested that optimized extraction technology was - DOI:10.3969/j.issn.1000-1565.2020.02.008响应面法优化紫叶李皮中总多酚的提取工艺及其抗炎活性高剑,孙焕然,国洪宾,陈保华,周程艳(河北大学 药学院,河北省药物质量分析控制重点实验室,河北 保定 071002)摘 要:为了优化紫叶李(Prunus cerasifera)皮中总多酚(PTP)提取工艺并研究其抗炎活性,在单因素实验的基础上,以总多酚得率为考察指标,乙醇体积分数、提取时间、提取温度、料液比为考察因素,采用Box-Behnken实验设计结合响应面分析法优化PTP的提取工艺,通过二甲苯致耳肿胀法、蛋清致足趾肿胀法测定PTP的抗炎活性.实验结果表明,PTP的最优工艺条件:乙醇体积分数69%、提取时间1.5 h、提取温度81 ℃、料液比1∶31(g∶mL),该工艺下得率为55.3 mg/g.PTP能抑制小鼠耳廓及大鼠足趾炎症.结论是该提取工艺稳定可靠,得率较高,且PTP具有明显的抗炎活性.关键词:紫叶李;总多酚;响应面法;提取工艺;抗炎活性中图分类号:R284.2 文献标志码:A 文章编号:1000-1565(2020)02-0161-10Extraction technology optimization of total polyphenols from Prunus cerasifera by response surface methodology and corresponding anti-inflammatory activity investigationGAO Jian,SUN Huanran,GUO Hongbin,CHEN Baohua,ZHOU Chengyan(Key Laboratory of Pharmaceutical Quality Control of Hebei Province,College of Pharmaceutical Science,Hebei University,Baoding 071002,China)Abstract: This work aims to optimize the extraction technology of total polyphenols(PTP)from Prunus cerasifera and study its anti-inflammatory activity.Based on the results of the single-factor experiment,using extraction rate of total polyphenols as an index,volume fraction of ethanol,extraction time,extraction temperature and solid-liquid ratio as factors,the Box-Behnken experimental design and the response surface methodology were applied to optimize the extraction technology of PTP.The anti-inflammatory activities of PTP were studied via ear swelling induced by xylene and toe swelling induced by albumen.The results showed that the optimum extraction conditions of PTP were as follows: volume fraction of ethanol 69%,extraction time 1.5 h,extraction temperature 81 ℃,solid-liquid ratio at 1∶31(g∶mL).Under these conditions,the yield of PTP was 55.3 mg/g.PTP could effectively inhibit the inflammation of ear swelling of mouse and toe swelling of rats.The results suggested that optimized extraction technology was - 收稿日期:2019-08-20 基金项目:河北省科技计划项目(B2015201016);保定市科学技术研究与发展指导计划项目(18ZF121) 第一作者:高剑(1995— ),男,安徽六安人,河北大学在读研究生,主要从事药物的提取、设计与合成. E-mail: 1741452250@qq.com 通信作者:周程艳(1976— ),女,河北唐山人,河北大学副教授,主要从事药理作用机制,药效学,中药药效和有效成分筛选的研究.E-mail:893927534@qq.com第2期高剑等:响应面法优化紫叶李皮中总多酚的提取工艺及其抗炎活性considered to be stable and reliable.The yield of PTP was higher in extraction rate,and it can be obvious anti-inflammatory activity.

Key words: Prunus Cerasifera, total polyphenols, response surface methodology, extraction technology, anti-inflammatory activity