关键词: 瑞香狼毒, 酒制, Ki-67, Caspase-3

Abstract: The difference in anti-tumor activity and toxic reaction in vivo between the crude medicine and liquor-saturated products was compared, and its preliminary anti-tumor mechanism was studied. Intragastric administration was used to compare and study the inhibitory effects of different concentrations of liquor-saturated and crude of Stellera chamaejasme. solution on mices subcutaneous S180 tumor. Immunohistochemical method was used to detect the expression levels of Ki-67 and Caspase-3 in tumor tissues, and to explore the antitumor mechanism of the drugs. There was a significant difference in tumor inhibition between liquor-saturated group and the crude drug group(P<0.05). Compared with the blank control group, the spleen index and thymus index of the wine group were not statistically difference(P<0.05). liquor-saturated Stellera chamaejasme can significantly down-regulate Ki-67 expression in tumor tissue of S180 tumor-bearing mice(P<0.01), and can significantly up-regulate the expression of Caspase-3(P<0.01). Liquor-saturated process can enhance the Stellera Chamaejasmes anti-tumor activity in S180 transplanted mice, and reduce the Stellera chamaejasmes impact on spleen index of mice. The anti-tumor- DOI:10.3969/j.issn.1000-1565.2020.04.009酒制对瑞香狼毒抗肿瘤活性及毒性反应的影响李红玲¹,宋美燕¹,马昊然¹,陆艺蓓¹,马晓莉²,徐艳娇¹(1. 河北大学 医学院,河北 保定 071000;2.河北大学 中医学院,河北 保定 071000)摘 要:实验对比了瑞香狼毒(stellera chamaejasme)生药和酒制品的体内抑瘤活性及毒性反应差异,并对其抑瘤机制进行了初步探讨.以灌胃的给药方式,对比不同浓度的酒制及生药瑞香狼毒溶液对小鼠S180皮下移植瘤的抑制作用.采用免疫组化法检测肿瘤组织Ki-67、Caspase-3的表达水平,探讨药物的抑瘤作用机制.结果表明:酒制瑞香狼毒组与生药组相比,抑瘤率有显著性差异(P<0.05);与空白对照组相比,酒制组脾指数、胸腺指数无统计学差异(P>0.05);酒制瑞香狼毒组S180荷瘤小鼠肿瘤组织中的Ki-67的表达显著下降(P<0.01),Caspase-3的表达增强(P<0.01).因此,酒制能增强瑞香狼毒对S180荷瘤小鼠的抑瘤活性,同时能减轻生药对小鼠脾指数的影响;其抗肿瘤作用机制可能与S180细胞中Ki-67的表达下降以抑制其增殖,Caspase-3的表达增强以促进细胞凋亡有关.关键词:瑞香狼毒;酒制;Ki-67;Caspase-3中图分类号:R332 文献标志码:A 【additional_page=420】文章编号:1000-1565(2020)04-0392-07Effects of liquor-saturated process on the Stellera chamaejasmes anti-tumor activity and toxicity LI Hongling¹, SONG Meiyan¹, MA Haoran¹, LU Yibei¹, MA Xiaoli², XU Yanjiao¹(1.Medical College,Hebei University,Baoding 071000,China;2.College of Traditional Chinese Medicine,Hebei University,Baoding 071000,China)Abstract: The difference in anti-tumor activity and toxic reaction in vivo between the crude medicine and liquor-saturated products was compared, and its preliminary anti-tumor mechanism was studied. Intragastric administration was used to compare and study the inhibitory effects of different concentrations of liquor-saturated and crude of Stellera chamaejasme. solution on mices subcutaneous S180 tumor. Immunohistochemical method was used to detect the expression levels of Ki-67 and Caspase-3 in tumor tissues, and to explore the antitumor mechanism of the drugs. There was a significant difference in tumor inhibition between liquor-saturated group and the crude drug group(P<0.05). Compared with the blank control group, the spleen index and thymus index of the wine group were not statistically difference(P<0.05). liquor-saturated Stellera chamaejasme can significantly down-regulate Ki-67 expression in tumor tissue of S180 tumor-bearing mice(P<0.01), and can significantly up-regulate the expression of Caspase-3(P<0.01). Liquor-saturated process can enhance the Stellera Chamaejasmes anti-tumor activity in S180 transplanted mice, and reduce the Stellera chamaejasmes impact on spleen index of mice. The anti-tumor- 收稿日期:2019-11-10 基金项目:河北省科技厅资助项目(17272502D) 第一作者:李红玲(1978—),女,河北容城人,河北大学实验师,主要从事中药抗肿瘤研究.E-mail:980374832@qq.com 通信作者:马晓莉(1967—),女,河北定兴人,河北大学教授,主要从事中药药物分析方向研究.E-mail:ma_ xiaoli@163.com第4期李红玲等:酒制对瑞香狼毒抗肿瘤活性及毒性反应的影响mechanism may be related to down-regulation of Ki-67 expression and enhancement of Caspase-3 expression. liquor-saturated Stellera chamaejasme can inhibit the proliferation of S180 tumor cells and promote their apoptosis.

Key words: Stellera chamaejasme, liquor-saturated, Ki-67, Caspase-3