关键词: 十八叔胺, 色谱固定相, 混合模式, 磷脂

Abstract: With N,N-dimethyloctadecan-1-amine(DMOA)as functional monomer, the polar embedded SiO₂-DMOA chromatographic stationary phase was prepared through ring-opening reaction. The successful fabrication of the SiO₂-DMOA stationary phase was characterized by thermogravimetric analysis and Fourier infrared spectroscopy. The retention mechanism research shows that the packed SiO₂-DMOA column indicates reversed-phase/hydrophilic mixed-mode retention mechanisms and the column efficiency can reach 81,100 N/m. The SiO₂-DMOA column can achieve high-efficiency separation of anilines, alkylbenzenes, amides, benzene derivatives, phenols and polycyclic aromatic hydrocarbons. The SiO₂-DMOA column was further applied to the separation of phospholipid standards. It can realize the effective separation and analysis of both phospholipid classes and phospholipid species. The SiO₂-DMOA column was further applied to the separation and analysis of soybean lecithin and phospholipids in lung adenocarcinoma cells, which also show a good separation potential.- DOI:10.3969/j.issn.1000-1565.2023.01.007十八叔胺修饰硅胶色谱固定相制备及分离性能研究车轩¹,刘德禄²,乔晓强²(1. 河北省生态环境监测中心,河北 石家庄 050000;2. 河北大学 药学院,河北 保定 071002)摘 要:以十八叔胺(DMOA)为功能单体,通过叔胺基团与环氧功能化的二氧化硅进行开环反应,制备十八叔胺极性嵌合的SiO₂-DMOA色谱固定相.傅里叶变换红外光谱和热重分析证明SiO₂-DMOA色谱固定相已成功制备.保留机制研究显示:填充SiO₂-DMOA色谱柱具有反相/亲水混合模式保留机制,其柱效可达81 100 N/m,并可实现苯胺类、烷基苯类、酰胺类、苯衍生物、苯酚类和多环芳烃类物质的有效分离.进一步将其用于磷脂标准品的分离,可实现磷脂类别和磷脂种类的有效分离分析.将其应用于大豆卵磷脂和肺腺癌细胞中磷脂的分离分析,显示了良好的分离潜力.关键词:十八叔胺;色谱固定相;混合模式;磷脂中图分类号:O657.7 文献标志码:A 文章编号:1000-1565(2023)01-0040-08Preparation and chromatographic properties of silica gel chromatographic stationary phase modified with N,N-dimethyloctadecan-1-amineCHE Xuan¹, LIU Delu², QIAO Xiaoqiang²(1. Hebei Ecological Environmental Monitoring Center, Shijiazhuang 050000, China; 2. College of Pharmacy, Hebei University, Baoding 071002, China)Abstract: With N,N-dimethyloctadecan-1-amine(DMOA)as functional monomer, the polar embedded SiO₂-DMOA chromatographic stationary phase was prepared through ring-opening reaction. The successful fabrication of the SiO₂-DMOA stationary phase was characterized by thermogravimetric analysis and Fourier infrared spectroscopy. The retention mechanism research shows that the packed SiO₂-DMOA column indicates reversed-phase/hydrophilic mixed-mode retention mechanisms and the column efficiency can reach 81,100 N/m. The SiO₂-DMOA column can achieve high-efficiency separation of anilines, alkylbenzenes, amides, benzene derivatives, phenols and polycyclic aromatic hydrocarbons. The SiO₂-DMOA column was further applied to the separation of phospholipid standards. It can realize the effective separation and analysis of both phospholipid classes and phospholipid species. The SiO₂-DMOA column was further applied to the separation and analysis of soybean lecithin and phospholipids in lung adenocarcinoma cells, which also show a good separation potential.- 收稿日期:2022-02-20 基金项目:河北省百名优秀创新人才支持计划(SLRC2019016) 第一作者:车轩(1971—),男,山西柳林人,河北省生态环境监测中心高级工程师,主要从事环境质量监测研究. E-mail: cx3025018@163.com 通信作者:刘德禄(1997—),男,吉林梅河口人,河北大学硕士研究生,主要从事色谱分析研究.E-mail: 945068682@qq.com乔晓强(1982—),男,河北宁晋人,河北大学教授,博士,主要从事色谱分离分析新材料研究. E-mail: hbuqiao@163.com第1期车轩等:十八叔胺修饰硅胶色谱固定相制备及分离性能研究

Key words: N,N-dimethyloctadecan-1-amine, chromatographic stationary phase, mixed-mode, phospholipids