谷氨酸铁的制备及表征

doi:10.3969/j.issn.1000-1565.2023.05.005

河北大学学报(自然科学版) ›› 2023, Vol. 43 ›› Issue (5): 476-483.DOI: 10.3969/j.issn.1000-1565.2023.05.005

谷氨酸铁的制备及表征

刘新硕,王真,李晴,杨文智,李海鹰

收稿日期:2022-06-29 出版日期:2023-09-25 发布日期:2023-10-25
通讯作者: 李海鹰(1973—)
作者简介:刘新硕(1998—),女,河北邢台人,河北大学在读硕士研究生,主要从事药物分析方向研究.
E-mail:1055177639@163.com
基金资助:
河北省自然科学基金资助项目(C2021201026);河北省大学生科技创新能力培育专项(22E50036D)

Preparation and characterization of glutamate iron chelate

LIU Xinshuo,WANG Zhen,LI Qing,YANG Wenzhi,LI Haiying

Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China

Received:2022-06-29 Online:2023-09-25 Published:2023-10-25

摘要/Abstract

摘要： 将谷氨酸(Glu)与三价铁离子(Fe³⁺)螯合,制备谷氨酸铁螯合物Glu-Fe(Ⅲ),并采用傅里叶变换红外光谱(FT-IR)、差示扫描量热(DSC)和热重(TGA)分析法对其结构进行表征,证实Glu与Fe³⁺成功发生螯合反应.采用间接碘量法测定Glu-Fe(Ⅲ)中的Fe³⁺质量分数,并以其为评价指标,通过单因素实验和Box-Benhnken设计(BBD),考察Glu和Fe³⁺的物质的量比及反应pH、时间、温度4个因素对制备Glu-Fe(Ⅲ)螯合物中Fe³⁺质量分数的影响.最终在Glu与Fe³⁺物质的量比为3∶5,反应pH为4.8、温度为50 ℃和反应时间为3 h的条件下,获得Fe³⁺质量分数为19.91%的Glu-Fe(Ⅲ)螯合物.制备出的Glu-Fe(Ⅲ)可作为同时补充谷氨酸和铁的营养强化剂.

关键词: 谷氨酸铁螯合物, 制备工艺, Box-Benhnken设计, 表征, 营养强化剂

Abstract: Glutamic acid(Glu)was reacted with trivalent iron ions(Fe³⁺)to prepare glutamate iron chelate ［Glu-Fe(Ⅲ)］. Glu-Fe(Ⅲ)was characterized by FT-IR, DSC, and TGA analysis. The Fe³⁺ content in Glu-Fe(Ⅲ)was determined by an indirect iodometric method and identified as an evaluation index for optimizing the preparation condition of Glu-Fe(Ⅲ). The effects of molar ratio of glutamic acid to iron, pH, time and temperature on the iron content of Glu-Fe(Ⅲ)were investigated by single factor method and Box-Benhnken design(BBD). The optimum preparation condition of Glu-Fe(Ⅲ)was Glu/Fe³⁺ molar ratio of 3∶5, pH 4.8, 50 ℃ and 3 h, and the iron content of Glu-Fe(Ⅲ)prepared under the optimized conditions was 19.91%. Glu-Fe(Ⅲ)could be a new iron and glutamic acid nutrient supplement.

Key words: glutamate iron chelate, preparation, Box-Benhnken design, characterization, nutrient supplements

中图分类号:

O634

刘新硕,王真,李晴,杨文智,李海鹰. 谷氨酸铁的制备及表征[J]. 河北大学学报(自然科学版), 2023, 43(5): 476-483.

LIU Xinshuo,WANG Zhen,LI Qing,YANG Wenzhi,LI Haiying. Preparation and characterization of glutamate iron chelate[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(5): 476-483.

参考文献

[1] 韦晓丽,周吉超,张晓伟.铁代谢及其在心力衰竭治疗中的作用机制研究进展[J].药学学报,2022,57(6):1584-1592. DOI: 10.16438/j.0513-4870.2022-0268.
[2] 林咏惟.铁缺乏的影响因素与预防干预的研究进展[J].福建医科大学学报,2020,54(6):465-470.
[3] 管玲娟,曹丛丛,屠飘涵,等.缺铁对肠道免疫功能的影响及新型补铁剂的研究进展[J].食品与发酵工业,2020,46(19):264-270.DOI: 10.13995/j.cnki.11-1802/ts.024455.
[4] ABDEL MOETY G A F A, ALI A M, FOUAD R, et al. Amino acid chelated iron versus an iron salt in the treatment of iron deficiency anemia with pregnancy: A randomized controlled study[J]. European Journal of Obstetrics & Gynecology and Reproductive Biology, 2017, 210: 242-246. DOI: 10.1016/j.ejogrb.2017.01.003.
[5] SANCHEZ J, VILLADA O A, ROJAS M L, et al. Effect of zinc amino acid chelate and zinc sulfate in the incidence of respiratory infection and diarrhea among preschool children in child daycare centers[J]. Biomed Research International, 2014, 34(1): 79-91. DOI: 10.1590/S0120-41572014000100011.
[6] LU Q, XU L, MENG Y B, et al. Preparation and characterization of a novel Astragalus membranaceus polysaccharide-iron(Ⅲ)complex[J]. International Journal of Biological Macromolecules, 2016, 93: 208-216. DOI: 10.1016/j.ijbiomac.2016.08.049.
[7] 房凤玲,丁劲松.L-谷氨酸的胃肠道保护作用机制及药物开发[J].药学学报,2017,52(4):517-523. DOI: 10.16438/j.0513-4870.2016-1016.
[8] BROSNAN J T, BROSNAN M E. Glutamate: a truly functional amino acid[J]. Amino Acids, 2013, 45(3): 413-418. DOI: 10.1007/s00726-012-1280-4.
[9] DUTTA G, RAJ GOSWAMI A, GHOSH T. Effects of stimulation of glutamate receptors in medial septum on some immune responses in rats[J]. Brain Research, 2013, 1538: 116-125. DOI: 10.1016/j.brainres.2013.09.038.
[10] JIAO N, WU Z L, JI Y, et al. L-glutamate enhances barrier and antioxidative functions in intestinal porcine epithelial cells[J]. Journal of Nutrition, 2015, 145(10): 2258-2264. DOI: 10.3945/jn.115.217661.
[11] ZHANG X Y, WANG D, ZHANG B, et al. Regulation of microglia by glutamate and its signal pathway in neurodegenerative diseases[J]. Drug Discovery Today, 2020, 25(6): 1074-1085. DOI:10.1016/j.drudis.2020.04.001.
[12] 李海鹰,杨祥禾,魏珍珍,等.羧甲基普鲁兰多糖螯合钙的制备及其功效评价[J].食品科学,2021, 42(15):143-149.DOI: 10.7506/spkx1002-6630-20200724-330.
[13] 国家药典委员会.中华人民共和国药典:二部[M].2020版.北京:中国医药科技出版社, 2020: 206.
[14] 李海鹰,段雨萌,贾蓓,等.CZE法同时测定复方二甲双胍片中盐酸二甲双胍和格列美脲含量[J].河北大学学报(自然科学版),2018,38(1):49-54.DOI: 10.3969/j.issn.1000-1565.2018.01.008.
[15] 邢伟平,万亚珍,杜小称,等.谷氨酸螯合锌的合成与表征[J].饲料研究,2018(1):53-57.DOI: 10.13557/j.cnki.issn1002-2813.2018.01.014.
[16] SHI Y Q, LU S Y, MA B, et al. A “one stop” thermal stabilizer, zinc arginine complex, with excellent comprehensive thermal stability effect on poly(vinyl chloride)[J]. Polymer Degradation and Stability, 2019, 167: 58-66. DOI: 10.1016/j.polymdegradstab.2019.06.016.
[17] 赵英虎,高莉,赵恒寿,等.谷氨酸甲氧苄啶合成物Glu-TMP的制备及检测[J].中国抗生素杂志,2013,38(1):50-52.DOI:10.13461/j.cnki.cja.005125.
[18] 黎新.谷氨酸热分解机理的研究[J].西南师范大学学报(自然科学版),1999,24(4):438-442.DOI:10.3969/j.issn.1000-5471.1999.04.012.
[19] 蒋楠,党乐平.液滴-微流体环境下L-谷氨酸的多晶型现象[J].化学工业与工程,2018,35(3):68-73.DOI:10.13353/j.issn.1004.9533.20161060.
[20] ZHOU R J, ZHANG M, LI J Y, et al. Optimization of preparation conditions for biochar derived from water hyacinth by using response surface methodology(RSM)and its application in Pb²⁺ removal[J]. Journal of Environmental Chemical Engineering, 2020, 8(5): 104198. DOI: 10.1016/j.jece.2020.104198. (

谷氨酸铁的制备及表征

Preparation and characterization of glutamate iron chelate

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	刘雪梅,郭珂欣,李金环,李慧敏,胡若晖. 季铵盐阳离子的Waugh型化合物的合成与表征[J]. 河北大学学报(自然科学版), 2022, 42(3): 265-272.
[2]	张保平,杨芳,马钟琛,郭美辰,刘运. 稻草木质素的硫酸法提取与表征[J]. 河北大学学报(自然科学版), 2016, 36(5): 474-479.
[3]	刘雪梅, 胡子艺, 刘蔓飞, 蒋芳. Waugh 型化合物(NH4)2(C5H6N)4MnMo9O32·8H2O的合成、晶体结构及表征[J]. 河北大学学报(自然科学版), 2015, 35(2): 153-158.
[4]	霍国燕,任鑫,霍纪,闫旭. ZnCr2-xFexO4系列化合物的合成及其性质表征[J]. 河北大学学报(自然科学版), 2012, 32(6): 619-624.
[5]	霍国燕,张成会,张红生,袁继承. 花棒状ZnO单晶体的生长与表征[J]. 河北大学学报(自然科学版), 2010, 30(5): 512-515.
[6]	白振华,范志东,程旭,彭英才. Si纳米线的气-液-固生长与结构表征[J]. 河北大学学报(自然科学版), 2009, 29(6): 582-586.
[7]	范志东,白振华,马蕾,彭英才. Si纳米线的Au催化固-液-固生长与结构表征[J]. 河北大学学报(自然科学版), 2009, 29(4): 357-360.