风积砂本土菌群诱导碳酸钙沉积过程的环境影响因素

doi:10.3969/j.issn.1000-1565.2026.03.008

摘要/Abstract

摘要： 微生物诱导碳酸钙沉积(microbial induced carbonate precipitation, MICP)过程可用于胶结风积砂颗粒以提高其抗风蚀性能,然而,外来单菌种如巴氏芽孢八叠球菌(Sporosarcina pasteurii)的活性易受沙化地区高温、高盐等恶劣环境的影响,从而制约MICP过程的效率.本文研究了利用沙化地区风积砂中本土细菌作为基于尿素水解的MICP过程媒介,并探索温度、环境酸碱度、尿素、钙盐以及钠盐浓度对菌群活性与MICP过程的影响,同时与S. pasteurii的表现进行对比.结果表明:风积砂中含有多种具有尿素水解功能的菌群,对河北张家口坝上地区而言,其菌种主要为Sporosarcina luteola和Sporosarcina psychrophila.风积砂筛选菌群在温度、钙盐和钠盐的耐受性方面表现更优,其MICP过程符合米氏方程,为MICP技术在沙化区域恶劣环境下的固沙及其他应用提供了更多可能性.

关键词: 微生物, 碳酸钙沉积, 尿素水解菌群, 沙化地区, 细菌活性, 米氏方程

Abstract: Microbial induced carbonate precipitation(MICP)is an emerging process that can be used to enhancewind erosion resistance of aeolian sands by cementing the grains. However, microbes,especially for the single foreignstrains, like Sporosarcinapasteurii,are likely to suffer from harsh and challenging environments of deserts including the high temperature, high salinity etc., which will inhibit the efficiency of MICP. The objective of this study is to investigate the feasibility of using native microbes from aeolian sands as a medium for urea-hydrolysis based MICP process. The effects of temperature, alkalinity and the- 引用格式:张文恺,杨术明,马永龙,等.基于EDEM的牧场推料机器人参数优化设计与试验［J］.河北大学学报(自然科学版),2026,46(3):225-236.引用格式:王丽亚,李光灿,安艳仓,等.风积砂本土菌群诱导碳酸钙沉积过程的环境影响因素［J］.河北大学学报(自然科学版),2026,46(3):299-309.DOI:10.3969/j.issn.1000-1565.2026.03.008风积砂本土菌群诱导碳酸钙沉积过程的环境影响因素王丽亚¹,李光灿¹,安艳仓¹,张自创²,李孟研¹,高云起²(1.石家庄铁道大学土木工程学院,道路与铁道工程安全保障教育部重点实验室,河北石家庄 050043;2.河北大学建筑工程学院,河北省基础设施防灾减灾与智能评估重点实验室,河北保定 071002)摘要:微生物诱导碳酸钙沉积(microbial induced carbonate precipitation, MICP)过程可用于胶结风积砂颗粒以提高其抗风蚀性能,然而,外来单菌种如巴氏芽孢八叠球菌(Sporosarcina pasteurii)的活性易受沙化地区高温、高盐等恶劣环境的影响,从而制约MICP过程的效率.本文研究了利用沙化地区风积砂中本土细菌作为基于尿素水解的MICP过程媒介,并探索温度、环境酸碱度、尿素、钙盐以及钠盐浓度对菌群活性与MICP过程的影响,同时与S. pasteurii的表现进行对比.结果表明:风积砂中含有多种具有尿素水解功能的菌群,对河北张家口坝上地区而言,其菌种主要为Sporosarcina luteola和Sporosarcina psychrophila.风积砂筛选菌群在温度、钙盐和钠盐的耐受性方面表现更优,其MICP过程符合米氏方程,为MICP技术在沙化区域恶劣环境下的固沙及其他应用提供了更多可能性.关键词:微生物;碳酸钙沉积;尿素水解菌群;沙化地区;细菌活性;米氏方程中图分类号:X1;X45 文献标志码:A 文章编号:1000-1565(2026)03-0299-11DOI:10.3969/j.issn.1000-1565.2026.03.008Effects of environmental factors on microbial carbonate precipitation induced by native bacteria enriched from aeolian sandWANG Liya¹, LI Guangcan¹, AN Yancang¹, ZHANG Zichuang², LI Mengyan¹, GAO Yunqi²(1. Key Laboratory of Roads and Railway Engineering Safety Control Ministry of Education, School of Civil Engineer, Shijiazhuang Tiedao University, Shijiazhuang 050043,China;2. Hebei Key Laboratory of Infrastructure Disaster Mitigation and Intelligent Assessment,College of Civil Engineering and Architecture,Hebei University, Baoding 071002, China)Abstract: Microbial induced carbonate precipitation(MICP)is an emerging process that can be used to enhancewind erosion resistance of aeolian sands by cementing the grains. However, microbes,especially for the single foreignstrains, like Sporosarcinapasteurii,are likely to suffer from harsh and challenging environments of deserts including the high temperature, high salinity etc., which will inhibit the efficiency of MICP. The objective of this study is to investigate the feasibility of using native microbes from aeolian sands as a medium for urea-hydrolysis based MICP process. The effects of temperature, alkalinity and the- 收稿日期:2025-08-28;修回日期:2025-11-17 基金项目:国家自然科学基金项目(52208357;52408359);河北省自然科学基金项目(E2023210018);河北省高等学校科学技术研究项目(BJK2024175) 第一作者:王丽亚(1991—),女,石家庄铁道大学讲师,主要从事环境岩土工程方向研究,E-mail:lwang@stdu.edu.cn 通信作者:高云起(1989—),男,河北大学副教授,主要从事生态岩土工程与岩土地震工程方向研究,E-mail:yqgao89@hbu.edu.cn 第3期王丽亚等:风积砂本土菌群诱导碳酸钙沉积过程的环境影响因素河北大学学报(自然科学版) 第46卷concentration of urea, calcium and sodium salts on the activity of selected bacteria were explored and compared with the performance of Sporosarcinapasteurii. MICP processes were also studied considering the effects of initial alkalinity and concentration of substrates. The results showed that a variety of ureolytic bacteria strains existed in aeolian sand from the harsh desert environment. Sporosarcinaluteol and Sporosarcinapsychrophila are the two main strains selected from aeolian sand collected from Zhangjiakou Bashang area, Hebei province. The activity was more stablefor the selected microbes which imposed more tolerance for temperature, calcium concentration and sodium salts. The MICP process fulfilled by the selected microbes, can be described by Michaelis-Menten kinetics. The high tolerance of the selected native microbes provides more possible engineering applications of MICP in the harsh environment of deserts.

Key words: microbial, carbonate precipitation, ureolytic bacteria, desert, bacterial activity, Michaelis-Menten kinetics

中图分类号:

王丽亚, 李光灿, 安艳仓, 张自创, 李孟研, 高云起. 风积砂本土菌群诱导碳酸钙沉积过程的环境影响因素[J]. 河北大学学报(自然科学版), 2026, 46(3): 299-309.

WANG Liya, LI Guangcan, AN Yancang, ZHANG Zichuang, LI Mengyan, GAO Yunqi. Effects of environmental factors on microbial carbonate precipitation induced by native bacteria enriched from aeolian sand[J]. Journal of Hebei University(Natural Science Edition), 2026, 46(3): 299-309.

参考文献

[1] Tunza. Save our soil[J]. Deserts & Drylands, 2006, 4(1): 4-5.
[2] 党晓宏,高永,虞毅,等.新型生物可降解PLA沙障与传统草方格沙障防风效益[J].北京林业大学学报, 2015, 37(3): 118-125. DOI:10.13332/j.1000-1522.20140245.
[3] Chang I, Prasidhi A K, Im J, et al. Soil treatment using microbial biopolymers for anti-desertification purposes[J]. Geoderma, 2015, 253: 39-47. DOI:10.1016/j.geoderma.2015.04.006.
[4] Wang G, Zhao W, Liu H, et al. Changes in soil and vegetation with stabilization of dunes in a desert-oasis ecotone[J]. Ecol Res, 2015, 30(4): 639-650. DOI:10.1007/s11284-015-1267-1.
[5] Al Qabany A, Soga K. Effect of chemical treatment used in MICP on engineering properties of cemented soils[J]. Géotechnique, 2013, 63(4): 331-339. DOI:10.1680/geot.sip13.p.022.
[6] He Z, Xu Y, Yang X, et al. Passivation of heavy metals in copper-nickel tailings by in-situ bio-mineralization: a pilot trial and mechanistic analysis[J]. Sci Total Environ, 2022, 838(Pt 4): 156504. DOI:10.1016/j.scitotenv.2022.156504.
[7] Cunningham A B, Sharp R R, Caccavo F, et al. Effects of starvation on bacterial transport through porous media[J]. Adv Water Resour, 2007, 30(6/7): 1583-1592. DOI:10.1016/j.advwatres.2006.05.018.
[8] Liu Y, Gao Y, He J, et al. An experimental investigation of wind erosion resistance of desert sand cemented by soybean-urease induced carbonate precipitation[J]. Geoderma, 2023, 429: 116231. DOI:10.1016/j.geoderma.2022.116231.
[9] Mitchell A C, Dideriksen K, Spangler L H, et al. Microbially enhanced carbon capture and storage by mineral-trapping and solubility-trapping[J]. Environ Sci Technol, 2010, 44(13): 5270-5276. DOI:10.1021/es903270w.
[10] Tian K, Wu Y, Zhang H, et al. Increasing wind erosion resistance of aeolian sandy soil by microbially induced calcium carbonate precipitation[J]. Land Degrad Dev, 2018, 29(12): 4271-4281. DOI:10.1002/ldr.3176.
[11] Dagliya M, Satyam N, Sharma M, et al. Experimental study on mitigating wind erosion of calcareous desert sand using spray method for microbially induced calcium carbonate precipitation[J]. J Rock Mech Geotech Eng, 2022, 14(5): 1556-1567. DOI:10.1016/j.jrmge.2021.12.008.
[12] 高玉峰,杨恩杰,何稼.基于微生物诱导碳酸钙沉积的防风固沙试验研究[J].河南科学, 2019, 37(1): 144-150. DOI:10.3969/j.issn.1004-3918.2019.01.024.
[13] Hodges T M, Lingwall B N. Case histories of full-scale microbial bio-cement application for surface erosion control[C] //Geo-Congress 2020. Minneapolis, Minnesota. American Society of Civil Engineers, 2020: 9-19. DOI:10.1061/9780784482834.002.
[14] Gat D, Ronen Z, Tsesarsky M. Soil bacteria population dynamics following stimulation for ureolytic microbial-induced CaCO₃ precipitation[J]. Environ Sci Technol, 2016, 50(2): 616-624. DOI:10.1021/acs.est.5b04033.
[15] Tobler D J, Cuthbert M O, Greswell R B, et al. Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite[J]. Geochim Cosmochim Acta, 2011, 75(11): 3290-3301. DOI:10.1016/j.gca.2011.03.023.
[16] 王逸杰,蒋宁俊.原位激发微生物成矿加固钙质砂的剪切与压缩特性研究[J].高校地质学报, 2021, 27(6): 662-669. DOI:10.16108/j.issn1006-7493.2020094.
[17] Erdmann N, Strieth D. Influencing factors on ureolytic microbiologically induced calcium carbonate precipitation for biocementation[J]. World J Microbiol Biotechnol, 2022, 39(2): 61. DOI:10.1007/s11274-022-03499-8.
[18] 蔡国柱.张家口地区风沙侵蚀危害及防护林骨干工程的配置[J].林业资源管理, 1987(2): 59-62. DOI:10.13466/j.cnki.lyzygl.1987.02.018.
[19] Zheng J, Jin X, Li Q, et al. Soil moisture variation and affecting factors analysis in the Zhangjiakou-Chengde district based on modified temperature vegetation dryness index[J]. Ecol Indic, 2024, 168: 112775. DOI:10.1016/j.ecolind.2024.112775.
[20] 王静爱,左伟.中国地理图集[M].北京:中国地图出版社, 2010.
[21] 王激清,李君,刘社平.冀西北地区农田土壤养分现状、变化与评价—以宣化县为例[J].干旱区资源与环境, 2010, 24(8): 158-163. DOI:10.13448/j.cnki.jalre.2010.08.027.
[22] 刘建平,李龙江,刘建晔,等.冀西北半干旱区农田土壤养分现状、变化与评价—以蔚县为例[J].河北北方学院学报(自然科学版), 2014, 30(1): 54-58. DOI:10.3969/j.issn.1673-1492.2014.01.011.
[23] Whiffin V S. Microbial CaCO₃ Precipitation for the production of Biocement[D]. Perth: Murdoch University, 2004.
[24] Meyer F D, Bang S S, Min S, et al. Microbiologically-induced soil stabilization: application of Sporosarcina pasteuriifor fugitive dust control[C] //Geo-Frontiers 2011. Dallas, Texas, USA. American Society of Civil Engineers, 2011: 4002-4011. DOI:10.1061/41165(397)409.
[25] Nikseresht F, Landi A, Sayyad G, et al. Sugarecane molasse and vinasse added as microbial growth substrates increase calcium carbonate content, surface stability and resistance against wind erosion of desert soils[J]. J Environ Manag, 2020, 268: 110639. DOI:10.1016/j.jenvman.2020.110639.
[26] Pisani W A, Jenness G R, Schutt T C, et al. Preferential adsorption of prominent amino acids in the urease enzyme of Sporosarcina pasteurii on arid soil components: a periodic DFT study[J]. Langmuir, 2022, 38(44): 13414-13428. DOI:10.1021/acs.langmuir.2c01854.
[27] Cuaxinque-Flores G, Aguirre-Noyola J L, Hernández-Flores G, et al. Bioimmobilization of toxic metals by precipitation of carbonates using Sporosarcina luteola: an in vitro study and application to sulfide-bearing tailings[J]. Sci Total Environ, 2020, 724: 138124. DOI:10.1016/j.scitotenv.2020.138124.
[28] 宋泉颖,徐俊,张宇.球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus)和嗜冷芽孢八叠球菌(Sporosarcina psychrophila)介导形成白云石晶体[J].微生物学通报, 2014, 41(10): 2155-2165. DOI:10.13344/j.microbiol.china.140026.
[29] Romero-Serrano M C, Bonilla-Salinas M, Durán-Hinojosa U, et al. Improving ureolysis in human urine by inoculating Amphibacillus bacteria selected by enrichment cultures[J]. Environ Technol, 2025, 46(23): 4641-4651. DOI:10.1080/09593330.2025.2516053.
[30] Bachmeier K L, Williams A E, Warmington J R, et al. Urease activity in microbiologically-induced calcite precipitation[J]. J Biotechnol, 2002, 93(2): 171-181. DOI:10.1016/S0168-1656(01)00393-5.
[31] Tsuda K. Studies on psychrotolerant endospore-forming bacteria for developing food preservation methods[D]. Kyoto: Kyoto University, 2016.
[32] Stocks-Fischer S, Galinat J K, Bang S S. Microbiological precipitation of CaCO₃[J]. Soil Biol Biochem, 1999, 31(11): 1563-1571. DOI:10.1016/S0038-0717(99)00082-6.
[33] 杨丰.生物诱导固化技术的优化研究[D].南京:河海大学, 2019.
[34] Lu Z, Qiu Y, Liu J,et al. Experimental study on microbially induced calcite precipitation for expansive soil stabilization[J]. Geomechanics and Engineering, 2023, 32(1): 85-96.
[35] 江钇垚,陈延博,卞怡,等.脲酶诱导矿化对铅、镉复合污染砂土固化修复的试验研究[J].浙江大学学报(工学版), 2025, 59(2): 332-341. DOI:10.3785/j.issn.1008-973X.2025.02.011.
[36] Wang W, Tan K W J, Chiang P L, et al. Impact of incorporating free calcium and magnesium on the heat stability of a dairy- and soy-protein-containing model emulsion[J]. Polymers, 2023, 15(22): 4424. DOI:10.3390/polym15224424.
[37] Lei S, Jia X, Zhao C,et al. A review of saline-alkali soil improvements in China: Efforts and their impacts on soil properties[J]. Agric Water Manag, 2025, 317: 109617. DOI:10.1016/j.agwat.2025.109617.
[38] Dejong J T, Gomez M G. State of the art: MICP soil improvement and its application to liquefaction hazard mitigation[C] //20th InternationaL conference on soil mechanics and geotechnical engineering. 2022: 405-508.
[39] Gomez M G, Graddy C M R, DeJong J T,et al. Stimulation of native microorganisms for biocementation in samples recovered from field-scale treatment depths[J]. J Geotech Geoenviron Eng, 2018, 144: 04017098. DOI:10.1061/(asce)gt.1943-5606.0001804.
[40] Ruyters S, Mertens J, Vassilieva E,et al. The red mud accident in ajka(Hungary): plant toxicity and trace metal bioavailability in red mud contaminated soil[J]. Environ Sci Technol, 2011, 45(4): 1616-1622. DOI:10.1021/es104000m.
[41] 黄小松.MICP修复重金属污染垃圾土的实施效果与试验参数研究[D].武汉:华中科技大学, 2022.
[42] Li L, Wu Y, Chong S et al. Influence of CO₂ migration from geological storage on the chemical composition of groundwater and monitoring indicators[J]. Environ Earth Sci, 2022, 81(3): 73. DOI:10.1007/s12665-022-10194-2. (