以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型，灌胃给予高、中、低剂量（0.4，0.2，0.1 g.kg-1）蚯蚓冻干粉，每天1次，同时饲喂高脂饲料，以仅饲喂高脂饲料组小鼠为对照，共饲养10周.提取小鼠肝脏总RNA，应用半定量RT-PCR技术检测实验组与对照组小鼠胆固醇逆转运相关基因卵磷脂胆固醇酰基转移酶（Lecithin cholesterol acyltransferase, LCAT）和脂蛋白酯酶（Lipoprotein Lipase, LPL）mRNA表达水平.结果表明，蚯蚓冻干粉极显著提高实验组小鼠LCAT mRNA表达水平（P<0.01），显著提高LPL mRNA表达水平（P<0.05），提示这是蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平的原因之一
Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemia mice models. The mice were treated with earthworm lyophilized powder at dosages of 0.4, 0.2 and 0.1 g/kg once a day and were fed with high-level lipid feeds at the same time for 10 weeks, the mice fed only with high-level lipid feeds were as the control. The total RNA of liver was extracted, and the changes of liver Lecithin cholesterol acyltransferase and Lipoprotein Lipase gene expression were analyzed by RT- PCR. The results indicated that earthworm lyophilized powder up-regulated Lecithin cholesterol acyltransferase and Lipoprotein Lipase mRNA expression markedly, suggesting it was one of the reasons for reducing serum cholesterol in hyperlipidemia mice of earthworm lyophilized powder.
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