为探讨P-选择素(Ps)在内毒素(LPS)致大鼠急性肺损伤(ALI)时肺组织中的表达及褪黑素(MT)对其表达的影响,将SD大鼠随机分为3组,对照(Control)组、模型(LPS)组、褪黑素干预(MT+LPS)组,采用气管内滴注脂多糖(LPS)的方法建立大鼠ALI模型,光镜下观察大鼠肺组织形态学改变,采用免疫组织化学染色技术、逆转录-聚合酶链反应(RT-PCR)和Western blot技术观察肺组织中P-选择素的表达变化.结果显示:模型组较对照组肺组织损伤严重,P-选择素的阳性表达明显增多(P<0.05);应用褪黑素干预后显著缓解上述变化(P<0.05).这说明褪黑素可能通过抑制P-选择素的表达发挥对急性肺损伤的保护作用.
To observe the expression of P-selectin(Ps)in lung tissues of acute lung injury(ALI)rat model induced by lipopolysaccharide(LPS)and examine the effect of melatonin(MT)to its expression all rats were randomly divided into three groups:Control group,LPS group and MT+LPS group.Lung tissues were imbedded by paraffin to observe morphological changes.The auther used immunohistochemical staining technique,reverse transcription-polymerase chain reaction(RT-PCR)and western blot method to- 收稿日期:2015-03-12 基金项目:河北省科技厅资助项目(08276101D-88) 第一作者:张智(1982—),男,河北衡水人,河北大学实验师,主要从事病理学基础实验研究.E-mail:zhzh313@163.com 通信作者:丁春华(1954—),女,河北保定人,河北医科大学教授,主要从事急性肺损伤研究.E-mail:dch54@sina.comdetect the expression of P-selectin.The results showed that compared with Control group,alveolar septum thickened significantly in LPS group,positive expression of P-selectin was very obvious(P<0.05);the administration of MT mitigated above changed significantly(P<0.05).The results suggested that MT possessed obvious protective effect on lung tissues during ALI,its protective mechanism might be related to the inhibition of the expression of P-selectin.
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