中华绒螯蟹蜕皮抑制激素多克隆抗体的制备及其表达分泌特征的组织学研究

  • 张凤娟 ,
  • 曹佳培 ,
  • 王鹏 ,
  • 穆淑梅 ,
  • 康现江
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  • 河北大学生命科学学院,河北省动物系统学与应用重点实验室,河北保定071002

网络出版日期: 2015-05-25

基金资助

国家自然科学基金资助项目,河北省自然科学基金资助项目,河北大学博士基金资助项目,河北省重点实验室运行补助项目

Polyclonal antibody preparation of molt-inhibiting hormone from Eriocheir sinensis and the characteristics of secretion in the aspect of histology

  • ZHANG Fengjuan ,
  • CAO Jiapei ,
  • WANG Peng ,
  • MU Shumei ,
  • KANG Xianjiang
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Online published: 2015-05-25

摘要

为了探讨中华绒螯蟹(Eriocheir sinensis)蜕皮抑制激素(molt-inhibiting-hormone,MIH)的表达分泌特征,利用分子生物学技术构建了表达载体pET-30a-MIH,并制备了MIH的兔抗多克隆抗体.制备出的多克隆抗体用于MIH在中华绒螯蟹视上神经节的免疫组织化学研究.结果显示,眼柄视上神经节分泌细胞中细胞类型1,2,3,4都参与了MIH的分泌,窦腺也呈现阳性反应.

本文引用格式

张凤娟 , 曹佳培 , 王鹏 , 穆淑梅 , 康现江 . 中华绒螯蟹蜕皮抑制激素多克隆抗体的制备及其表达分泌特征的组织学研究[J]. 河北大学学报(自然科学版), 2015 , 35(3) : 272 -277 . DOI: 10.3969/j.issn.1000-1565.2015.03.009

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