为了有效保护和利用野生华北蓝盆花(Scabiosa tschiliensis Grunning)资源,通过离体培养技术建立了华北蓝盆花再生体系.研究结果表明:以子叶为外植体,诱导愈伤组织较适宜的培养基为M S+ TDZ1.0 mg/L,诱导率达82.74％;诱导不定芽的培养基为MS+6-BA 4.0 mg/L,诱导率为86.30％;生根培养基为MS+IBA 2.0 mg/L,生根率为73.85％.愈伤组织、野生植株总黄酮含量较高,其醇提物具有较强的抗氧化活性,对DPPH自由基具有很高的清除率.
[1] 王国英, 薛培凤, WANG Guo-ying, XUE Pei-feng. 蒙药蓝盆花及其同属植物的研究进展 [J]. 内蒙古医学院学报 2009.doi:10.3969/j.issn.1004-2113.2009.05.013
[2] 高丽华, 胡显文. 分光光度法测定水母雪莲细胞培养物中的总黄酮 [J]. 生物技术通讯 2003.doi:10.3969/j.issn.1009-0002.2003.02.011
[3] 赵宗孝, 好斯巴特, 汤文莉, 张昕原, 巴根那. 五种中药材清除自由基作用的实验研究 [J]. 时珍国医国药 2004.doi:10.3969/j.issn.1008-0805.2004.02.004
[4] 宋韵霏, 王俊丽, 毕凯丽, 张璐, 李建飞, SONG Yunfei, WANG Junli, BI Kaili, ZHANG Lu, LI Jianfei. 胭脂花的组织培养与植株再生 [J]. 河北大学学报（自然科学版） 2012.
[5] 王俊丽, 陆远, 刘坤, 许东婷, 刘名飞, WANG Jun-li, LU Yuan, LIU Kun, XU Dong-ting, LIU Ming-fei. 华北大黄愈伤组织培养及土大黄苷含量研究 [J]. 河北大学学报(自然科学版) 2011.doi:10.3969/j.issn.1000-1565.2011.02.018
[6] Zheng Q, Koike K, Han LK, Okuda H, Nikaido T. New biologically active triterpenoid saponins from Scabiosa tschiliensis. [J]. Journal of natural products 2004, 4(4).
[7] Tan SK, Pippen R, Yusof R, Ibrahim H, Rahman N, Khalid N. Simple one-medium formulation regeneration of fingerroot [Boesenbergia rotunda (L.) Mansf. Kulturpfl.] via somatic embryogenesis [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2005, 6(6).
[8] Ahire, Mahendra Laxman, Ghane, Savaliram Goga, Lokhande, Vinayak Haribhau, Suprasanna, Penna, Nikam, Tukaram Dayaram.. Micropropagation of Uraria picta through adventitious bud regeneration and antimicrobial activity of callus [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2011, 4(4).
[9] DAI Hongyan, ZHANG Zhihong, GUO Xiuwu. Adventitious bud regeneration from leaf and cotyledon explants of Chinese hawthorn (Crataegus pinnatifida Bge.var.major N.E.Br.) [J]. In Vitro Cellular & Developmental BiologyPlant 2007, 43(01).
[10] Guo B, Gao M, Liu CZ. In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir [J]. Plant Cell Reports 2007, 3(3).
[11] Wang, Junli, Wang, Qian, Wang, Jue, Lu, Yuan, Xiao, Xuan, Gong, Weizhen, Liu, Jikai. Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet [J]. Physiology and Molecular Biology of Plants 2009, 4(4).
[12] Wang, Junli, Wang, Jue, Liu, Kun, Xiao, Xuan, Gong, Weizhen, Lu, Yuan, Liu, Mingfei, Xu, Dongting.. An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2010, 5(5).
