以葡萄糖为主要碳源,采用城市污水处理厂曝气池絮状活性污泥为接种污泥,在自制的序批式反应器(sequence batch reactor,SBR)中持续培养45 d,通过提高SBR反应器中水力剪切力、增加污泥沉降速度和提高活性污泥的有机负荷等手段不断改变SBR反应器的运行条件,发现当化学需氧量(chemical oxygen demand,COD)负荷为3.97 kg/(m3·d),SBR反应器中表面上升气体流速为0.021 8 m/s时,培养出的好氧颗粒污泥直径可达2～5 mm,污泥浓度值和污泥容积指数(sludge volume index,SVI)分别为4 200 mg/L和50 mL/g,SBR反应器中培养出的好氧颗粒污泥对COD,总氮(total nitrogen,TN),总磷(total phosphorus,TP)的去除率分别可达到93％,82％和82％.本实验通过对污泥颗粒化转化过程的研究表明:从絮状活性污泥向颗粒污泥转化是一种逐步形成的过程,整个45 d的培养过程可划分为4个不同阶段,即接种活性污泥微生物相互黏附阶段、细菌聚集体的稳定阶段、细菌集合体的成熟阶段和好氧颗粒污泥最终形成4个阶段.
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