为了获得适于标记寡核苷酸适配子的胶体金,对制备工艺条件进行了优化:20 mL 0.1 g/L氯金酸溶液中添加10 g/L柠檬酸三钠500 μL,pH=3.0,初始沸腾时间小于4 min,加入还原剂后反应时间为6 min.通过紫外可见光谱扫描和透射电镜对其性能进行鉴定,获得了分散均匀、直径15～20 nm的胶体金颗粒,可准确标记核酸适配子,且将胶体金标记的链霉素适配子与半抗原小分子链霉素进行反应,可对链霉素进行定性和半定量检测,满足利用胶体金标记适配子检测靶物质的需要.
[1] 宋宏新, 符海英, 薛海燕, 张玥, 邹联柱, SONG Hong-xin, FU Hai-ying, XUE Hai-yan, ZHANG Yue, ZOU Lian-zhu. 两种胶体金制备方法的比较研究 [J]. 河南工业大学学报（自然科学版） 2012.doi:41-1378/N.20120208.0844.013
[2] 杨玉新, 叶阳, 周有祥, 王小红, YANG Yu-xin, YE Yang, ZHOU You-xiang, WANG Xiao-hong. 四种化学还原法制备胶体金的比较研究 [J]. 湖北农业科学 2011.doi:10.3969/j.issn.0439-8114.2011.03.013
[3] 彭剑淳, 刘晓达, 丁晓萍, 付占江, 王全立, PENG Jian-chun, LIU Xiao-da, DING Xiao-ping, FU Zhan-jiang, WANG Quan-li. 可见光光谱法评价胶体金粒径及分布 [J]. 军事医学科学院院刊 2000.doi:10.3969/j.issn.1674-9960.2000.03.014
[4] 汪琳, 周琦, 赖平安, 张鹤晓. 免疫胶体金技术及其在检验检疫中的应用 [J]. 检验检疫科学 2004.doi:10.3969/j.issn.1674-5354.2004.04.019
[5] TUERK C, GOLD L. Systematic evolution of ligands by exponential enrichment:RNA Ligands to bacteriophage T4 DNA polymerase [J]. SCIENCE 1990, 249.
[6] JOHN G, BRUNO. A review of therapeutic aptamer conjugates with emphasis on new approaches [J]. PHARMACEUTICALS 2013, 6(03).
[7] Barthelmebs, L, Jonca, J, Hayat, A, Prieto-Simon, B, Marty, J. L.. Enzyme-Linked Aptamer Assays (ELAAs), based on a competition format for a rapid and sensitive detection of Ochratoxin A in wine. [J]. Food Control 2011, 5(5).
[8] Zhu, Y., Chandra, P., Ban, C., Shim, Y.-B.. Electrochemical Evaluation of Binding Affinity for Aptamer Selection Using the Microarray Chip [J]. Electroanalysis 2012, 5(5).
[9] SONG K M, LEE S, BAN C. Aptamers and their biological applications [J]. SENSORS 2012, 12.
[10] 宋宏新, 邹联柱, 李韵, 李红心. 纳米胶体金的制备及其抗体标记研究 [J]. 2011.
[11] 韩刚毅. 胶体金标记抗体技术 [J]. 生物化学与生物物理进展 1989, 16(01).
[12] Zhou, N., Zhang, J., Li, C., Tian, Y., Wang, J.. Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey [J]. Talanta: The International Journal of Pure and Applied Analytical Chemistry 2013.
[13] NIKHIL R, JANA, YING Y J. Synthesis of functionalized Au nanoparticles for protein detection [J]. Advanced Materials 2008, 20.
[14] Li HX, Rothberg L. Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles [J]. Proceedings of the National Academy of Sciences of the United States of America 2004, 39(39).
[15] Li, HX, Rothberg, LJ. Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction [J]. Journal of the American Chemical Society 2004, 35(35).
[16] Cvak, B., Pum, D., Molinelli, A., Krska, R.. Synthesis and characterization of colloidal gold particles as labels for antibodies as used in lateral flow devices [J]. The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry 2012, 8(8).
[17] Song, K.-M., Jeong, E., Jeon, W., Cho, M., Ban, C.. Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods [J]. Analytical and bioanalytical chemistry 2012, 6(6).
