测定了9种拟步甲的16S rDNA部分基因序列,并与GenBank中的1种步甲的基因序列作同源性比较,计算其核苷酸使用频率并构建了分子系统树.在获得的435 bp的序列中,A+T约占74.4%,颠换(transversion)取代的速率大于转换(transition)取代的速率,其中277个核苷酸位点存在变异.结果表明:属内种间的碱基序列差异范围为3.4%～6.2%;族内属间为9.4%～11.0%;科内族间为10.8%～17.7%;科与科间的差异达到46.7%～50.3%.分子系统树表明:拟步甲科为一单系群,其中琵甲族较为进化,漠甲族与漠王族相对原始;琵甲族与土甲族的亲缘关系较近;漠甲族、漠王族与鳖甲族的亲缘关系较近.本结果与传统的分类观点相吻合.
[1] 成新跃, 周红章, 张广学, CHENG Xin-Yue, ZHOU Hong-Zhang, ZHANG Guang-Xue. 分子生物学技术在昆虫系统学研究中的应用 [J]. 动物分类学报 2000.doi:10.3969/j.issn.1000-0739.2000.02.001
[2] 印红, 刘晓丽, 王彦芳, 张道川, 赵晓瑜. 一种改进的昆虫基因组DNA的提取方法 [J]. 河北大学学报(自然科学版) 2002.doi:10.3969/j.issn.1000-1565.2002.01.021
[3] 常青, 周开亚, CHANG Qing, ZHOU Kai-Ya. 分子进化研究中系统发生树的重建 [J]. 生物多样性 1998.doi:10.3321/j.issn:1005-0094.1998.01.010
[4] 任国栋, 于有志. 中国荒漠半荒漠的拟步甲科昆虫 [M]. 保定:河北大学出版社 1999.
[5] Brown W M, GEORGE J R, WILSON A C. Rapid evolution of animal mitochondrial DNA [J]. Proceedings of the National Academy of Sciences of the United States of America 1979, 76.
[6] Avise J C, AMOLD J, BALL R M. Intraspecific phylogeography: the mitochondrial bridge between population genetics and systematics [J]. Annual Review of Ecology and Systematics 1987, 18.
[7] Moritz C, DOWLING T E, BROWN W M. Evolution of animal mitochondrial:relevance for population biology and systematics [J]. Annual Review of Ecology and Systematics 1987, 187.
[8] Kambhampati S, Kjer KM, Thorne BL. Phylogenetic relationship among termite families based on DNA sequence of mitochondrial 16S ribosomal RNA gene. [J]. Insect Molecular Biology 1996, 4(4).
[9] Gimeno C, Belshaw R, Quicke DLJ. Phylogenetic relationships of the Alysiinae/Opiinae (Hymenoptera: Braconidae) and the utility of cytochrome b, 16S and 28S D2 rRNA. [J]. Insect Molecular Biology 1997, 3(3).
[10] Dowton M, Austin AD, Antolin MF. Evolutionary relationships among the Braconidae (Hymenoptera: Ichneumonoidea) inferred from partial 16S rDNA gene sequences [J]. Insect Molecular Biology 1998, 2(2).
[11] Kambhampati S., Mackauer M., Volkl W.. Phylogenetic relationships among genera of Aphidiinae (Hymenoptera : Braconidae) based on DNA sequence of the mitochondrial 16S rRNA gene [J]. Systematic Entomology 2000, 4(4).
[12] Srinivas Kambhampati, Ralphe. Charlton. Phylogenetic relationship among Libellula, Ladona and Plathemis (Odonata: Libellulidae) based on DNA sequence of mitochondrial 16S rRNA gene [J]. Systematic Entomology 1999, 1(1).
[13] Yeh W B, YANG C T, HUI C F. Phylogenetic relationships of the Tropiduchidae-group(Homoptera:Fulgoroidea)of planthoppers inferred through nucleotide sequences [J]. Zoological Studies 1998, 37(01).
[14] FANG Q W, BLACK IV C, BLOCKER H D. A phylogeny of new world Deltocephalus-like leafhopper genera based on mitochondrial 16S ribosomal DNA sequences [J]. Molecular Phylogenetics and Evolution 1993, 2.
[15] 张亚平. 从DNA序列到物种树 [J]. 1996.
[16] 黄原. 分子系统学--原理、方法及应用 [M]. 北京:中国农业出版社 1998.
[17] 叶文斌, 杨仲图, 许祖法. 线粒体16S核糖体去氧核糖核酸序列分析蜡蝉总科类缘关系 [A]. 台湾大学昆虫学系, 台湾省博物馆 1999.
[18] Crowson R A. The natural classification of the family of Coleoptera [M]. London: Nathaniel Lionyd 1955.
[19] RICHARDS O W, DAVIES R G. Imms'outlines of entomology(Sixth Edition) [M]. Chapman and Hall Ltd 1987.
[20] ZhengLY, GUI H. Insect Classification(2) [M]. 南京:南京师范大学出版社 1999.
[21] WATT J C. A revised subfamily classification of Tenebriondae(Coleoptera) [J]. New Zealand Jour Zool 1974, 1.
[22] Reitter E. Bestimmungs-Schluse fur die Unterfanilien und Tribus der Palaarktischen Tenebrionidae [J]. Wiener Entomologische Zeitung 1917, 36(3-5).
[23] Reitter E. Bestimmung-Schluse fur die Unterfamilien: Belopinae, Borinae, Tenebrionini und Coelometopinae der Tenebrioninen(Col.) [J]. Best Tab Eur Col 1920, 87.
