以单因素和均匀设计实验为手段优化了茵陈多糖的提取工艺条件,采用苯酚硫酸法测定多糖的含量,计算每次实验纯多糖的得率,然后以纯多糖的产率为指标,采用SPSS软件对提取温度、提取液固比、提取时间3个因素进行多元线性回归分析,得出优化工艺条件,并进行验证.得到茵陈多糖的优化提取条件为:提取温度100℃、提取液固比50∶1、提取时间80 min.验证实验平均得率为2.25％,预测值为2.26％,二者十分接近,证明得到的优化工艺条件是可靠的.
[1] 范丽梅, 徐立堃, 张兰, 胡春玲, 林常青, 李莉群, FAN Limei, XU Likun, ZHANG Lan, HU Chunling, LIN Changqing, LI Liqun. 茵陈中多糖组分的提取及分析 [J]. 中国现代医生 2012.
[2] 陈晓清, CHEN Xiao-qing. 鱼腥草与茵陈多糖提取物的抗菌活性 [J]. 微量元素与健康研究 2007.doi:10.3969/j.issn.1005-5320.2007.05.007
[3] 赫长胜, 李源渊, 岳洪义, 薛峰, 刘健, HE Changsheng, LI Yuanyuan, YUE Hongyi, XUE Feng, LIU Jian. 茵陈多糖对梗阻性黄疸幼鼠肝脏纤维化的保护作用研究 [J]. 中国医药导报 2012.doi:10.3969/j.issn.1673-7210.2012.08.010
[4] 范丽梅, 徐立堃, 张兰, 胡春玲, 林常青, 李莉群, FAN Limei, XU Likun, ZHANG Lan, HU Chunling, LIN Changqing, LI Liqun. 茵陈多糖对妊娠胆汁淤积大鼠肝脏氧化损伤的保护作用研究 [J]. 中国医药导报 2013.
[5] 曹锦花. 茵陈的化学成分和药理作用研究进展 [J]. 2013.
[6] 范丽梅, 胡春玲, 谢琼, 徐力堃, 邵光. 茵陈多糖对妊娠胆汁淤积大鼠Th1/Th2细胞免疫平衡的影响 [J]. 中国热带医学 2013.
[7] HE Changsheng, YUE Hongyi, XU Jian. Protective effects of capillary artemisia polysaccharide on oxidative injury to the liver in rats with obstructive jaundice [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE 2012, 4(04).
[8] Guofang Feng, Xiaoqing Wang, Changxuan You. Antiproliferative potential of Artemisia capillaris polysaccharide against human nasopharyngeal carcinoma cells [J]. Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides 2013, 2(2).
[9] Lee JH, Park EK, Uhm CS, Chung MS, Kim KH. Inhibition of Helicobacter pylori Adhesion to Human Gastric Adenocarcinoma Epithelial Cells by Acidic Polysaccharides from Artemisia capillaris and Panax ginseng. [J]. Planta medica: Natural products and medicinal plant research 2004, 7(7).
