建立了使用凝胶渗透色谱-目相萃取-高效液相色谱(GPC-SPE-HPLC)测定油脂及油炸食品中的4种常见酚类抗氧化剂没食子酸丙酯(PG)、叔丁基对苯二酚(TBHQ)、叔丁基对羟基茴香醚(BHA)和二丁基羟基甲苯(BHT)的方法.试样经乙酸乙酯-环己烷(V(乙酸乙酯)∶V(环已烷)=1∶1)和乙腈漩涡振荡萃取,凝胶渗透色谱( GPC)和HLB固相萃取柱净化,以乙腈-体积分数为0.1％甲酸水溶液为流动相,以BDS HYPERSIL C18柱作为分离柱,在检测波长280 nm下用紫外检测器检测.方法的检出限为0.6～1.0 mg/kg(油脂样品)和0.3～0.5 mg/kg(油炸样品),定量限为2～3mg/kg(油脂样品)和1～1.7 mg/kg(油炸样品),线性相关系数r＞0.998,平均回收率为80.3％～94.0％(油脂样品)和80.3％～93.4％(油炸样品)；相对标准偏差(RSD)为3.22％～8.80％(油脂样品)和2.74％～9.62％(油炸样品).该方法选择性好,灵敏度高,检出限低,能够成功地应用于油脂及油炸食品中4种常见酚类抗氧化剂的检测.
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