采用反相高效液相色谱法检测人体血浆中替勃龙的含量.以5倍样品体积的乙腈萃取2次,经氮气吹干,甲醇溶解后,上机分析.采用Promosil-C18色谱柱(4.6 mm×250 mm,5μm),流动相:甲醇-乙腈-水,流速:1.0 mL/min,进样量:10μL,检测波长:204 nm,柱温.25℃.在优化的色谱条件下,替勃龙在0.5～250.0 μg/mL内线性关系良好(r=0.999 9),最低检测限为0.05 μg/mL,平均回收率(n=3)为92.7％,RSDs小于1.6％.本方法简单,灵敏,准确,适合于体内替勃龙含量的检测与分析.
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