建立了一种高效毛细管电泳方法,用于缬沙坦和笨磺酸氨氯地平的同时分析:以30 mmol/L磷酸纳缓冲液(pH=7.47)作为电解质溶液,检测波长237 nm,分离电压15 kV,高度差进样10 s.同时进行了方法学验证:缬沙坦在0.050 4～0.504 0 mg/mL内线性良好(r=0.999 8,n=6),迁移时间和峰面积的相对标准偏差(RSD)分别为0.3％和1.3％(n=6),回收率为98.98％～99.79％;苯磺酸氨氯地平在0.050 4～0.504 0mg/mL内线性良好(r=0.999 6,n=6),迁移时间和峰面积的RSD为0.6％和1.4％(n=6),回收率为98.22％～99.46％.通过上述高效毛细管电泳法,缬沙坦和苯磺酸氨氯地平在15.0 min内分离良好,分离度(R=29.8).此方法快速,灵敏,实用.
[1] 胡在林, Hu Zailin. 反相高效液相色谱法测定苯磺酸氨氯地平胶囊的含量和有关物质 [J]. 中国药业 2011.doi:10.3969/j.issn.1006-4931.2011.12.017
[2] Rosenthal T, Gavras I. Fixed-drug combinations as first-line treatment for hypertension. [J]. Progress in Cardiovascular Diseases 2006, 6(6).
[3] Panderi IE. Simultaneous determination of benazepril hydrochloride and hydrochlorothiazide in tablets by second-order derivative spectrophotometry. [J]. Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis 1999, 2(2).
[4] 邓庆文, 赵旭丽, 郭洁, 黄毕生. 流动注射-化学发光法测定苯磺酸氨氯地平 [J]. 2011.
[5] Zakrzewski Jakubiak M, de Denus S, Leblanc MH, White M, Turgeon J. Enantioselective quantification of carvedilol in human plasma by HPLC in heavily medicated heart failure patients. [J]. Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis 2010, 4(4).
[6] 刘雪松, 刘伟, 门金玉, 闫冬, 高静. 高效液相色谱-串联质谱法测定人血浆中的缬沙坦 [J]. 2011.
[7] Kesting JR, Huang J, Sorensen D. Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model. [J]. Journal of Pharmaceutical and Biomedical Analysis 2010, 3(3).
