为了获得耐盐性有所提高的转AlNHX1基因大豆后代材料,以已获得的转AlNHX1基因的6个株系中的3个株系后代为研究对象,通过分别对这3个株系转基因大豆各后代进行PCR分子检测并结合耐盐性鉴定,以分析外源基因在转基因大豆中遗传稳定性和耐盐性.结果表明:AlNHX1基因在转基因植株的后代中遗传；选取3个株系中部分阳性植株做耐盐性检测,结果表明:转基因大豆耐盐性状均好于野生型大豆.在150 mmol/L NaCl胁迫下,转基因大豆叶片中维持了相对较高的K+/Na+比值,相对含水量较野生型提高了9％,而渗透势降低了39％,表明转基因大豆具有较好的吸水和保水能力；在盐胁迫下,超氧化物歧化酶( SOD)与过氧化物酶(POD)活性较野生型大豆分别提高了45％与69％.综上,通过耐盐筛选获得的转AlNHX1基因大豆具有较强的耐盐性.
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