为有效保护和利用华北大黄野生资源,以野生华北大黄为材料,探讨了不同种类、不同质量浓度的植物生长调节剂对其愈伤组织诱导及增殖效应,并采用高效液相色谱对愈伤组织及野生植株中土大黄苷含量进行了分析.结果表明:MS培养基中单独添加不同质量浓度的6-BA,不利于愈伤组织的增殖;培养基中单独添加不同质量浓度的NAA和2,4-D,对愈伤组织的增殖有一定作用;6-BA分别与低质量浓度NAA及2,4-D配合使用时,对愈伤组织的增殖效应十分明显.3种不同类型的愈伤组织中均检出土大黄苷,其中2种愈伤组织土大黄苷的质量分数高达1.45%～1.54%,为野生植株的3.63～3.85倍.
[1] 王爱芹, 李军林, 吴祖泽. 华北大黄中芪类成分的研究 [J]. 中草药 2001.doi:10.3321/j.issn:0253-2670.2001.10.006
[2] 《中国科学院中国植物志》编辑委员会. 中国植物志 [M]. 北京:科学出版社 2004.
[3] 郑俊华, 西泽信, 山岸乔. 三种非正品大黄中34种化学成分的高效液相色谱法定量分析 [J]. 北京医科大学学报 1989, 21(04).
[4] 徐颖, 郭增军, 谭林. 土大黄苷研究进展 [J]. 2009.
[5] Kang SM, Min JY, Kim YD, Kang YM, Park DJ, Jung HN, Kim SW, Choi MS. Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2006, 1(1).
[6] Jayapaul K, Kishor PBK, Reddy KJ. Production of pyrroloquinazoline alkaloid from leaf and petiole-derived callus cultures of Adhatoda zeylanica [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2005, 5(5).
[7] WANG Junli, LIAO Xiangru, ZHANG Hongmei. Accumulation of chlorogenic acid in cell suspension cultures of Eucommia ulmoides Oliv [J]. Plant Cell Tissue and Organ Culture 2003, 74(02).
[8] Makunga NP, van Staden J. An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L. [J]. Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants 2008, 1(1).
[9] Murugesan Dhandapani, Doo Hwan Kim, Seung-Beom Hong. Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus [J]. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association 2008, 1(1).
[10] Guo B, Gao M, Liu CZ. In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir [J]. Plant Cell Reports 2007, 3(3).
[11] 胡人伦, 朱宝成. 药用植物河北大黄叶片的组织培养研究 [J]. 1991.
[12] 逯海龙, 韩义欣, 董文静. HPLC法测定栽培河套大黄中土大黄苷的含量 [J]. 2007.
