为了研究中华锯齿米虾卵黄蛋白的性质,利用聚丙烯酰胺凝胶电泳,采用不同的染色方法对其进行分析鉴定,并采用电泳洗脱法从中华锯齿米虾成熟卵巢中将其分离纯化.研究结果表明,中华锯齿米虾成熟卵巢中的卵黄蛋白为糖脂复合蛋白,该蛋白分子质量为434 ku.利用SDS-聚丙烯酰胺凝胶电泳技术分析该卵黄蛋白由3个主要亚基组成,分子质量分别为108,89,78 ku.
[1] 谢松, 安建宏, 梁晓辉, 郭成秀, 武金霞, 王丙莲, 张园, XIE Song, AN Jian-hong, LIANG Xiao-hui, GUO Cheng-xiu, WU Jin-xia, WANG Bing-lian, ZHANG Yuan. 克氏原螯虾(Procambius clarkii)卵黄蛋白的部分生化性质 [J]. 河北大学学报(自然科学版) 2006.doi:10.3969/j.issn.1000-1565.2006.02.021
[2] 穆淑梅, 康现江, 李彦芹, 郭明申, 温秀荣, MU Shu-mei, KANG Xian-jiang, LI Yan-qin, GUO Ming-shen, WEN Xiu-rong. 日本沼虾卵黄磷蛋白生化性质分析 [J]. 河北农业大学学报 2006.doi:10.3969/j.issn.1000-1573.2006.05.017
[3] 柳峰松, 武金霞, 谢松, 房宝玲, 黄增瑞, 吴琛. 成熟中国对虾(Penaeus chinensis)卵巢中卵黄蛋白的纯化 [J]. 河北大学学报(自然科学版) 2001.doi:10.3969/j.issn.1000-1565.2001.04.017
[4] SHECHTER A, AFLALO E D, DAVIS C. Expression of the reproductive female-specific vitellogenin gene in endocrinologically induced male and intersex Cherax quadricarinatus crayfish [J]. Biology of Reproduction 2005, 73.
[5] Avarre JC., Michelis R., Tietz A., Lubzens E.. Relationship between vitellogenin and vitellin in a marine shrimp (Penaeus semisulcatus) and molecular characterization of vitellogenin complementary DNAs [J]. Biology of Reproduction 2003, 1(1).
[6] E. COCCIA, E. DE LISA, C. DI CRISTO, DI COSMO, M. PAOLUCCI. Effects of Estradiol and Progesterone on the Reproduction of the Freshwater Crayfish Cherax albidus [J]. The Biological Bulletin 2010, 1(1).
[7] Spano L, Tyler CR, van Aerle R, Devos P, Mandiki SNM, Silvestre F. Effects of atrazine on sex steroid dynamics, plasma vitellogenin concentration and gonad development in adult goldfish (Carassius auratus) [J]. Aquatic Toxicology 2004, 4(4).
[8] ICHIRO KAWAZOE, SAFIAH JASMANI, TUNG-WEI SHIH, YUZURU SUZUKI, KATSUMI AIDA. Purification and characterization of vitellin from the ovary of kuruma prawn, Penaeus japonicus [J]. Fisheries Science 2000, 2(2).
[9] QUINTIO E T, HARA A, YAMAUCHI K. Isolation and characterization of vitellin from the ovary of Penaeus monodon [J]. Invertebrate Reproduction &-Development 1990, 17(03).
[10] TOM M, FINGERMAN M, HAYES T K. A comparative study of the ovarian proteins from two penaeus shrimp, Penaeus semisulcatus de Hann and Penaeus vannamei (Boone) [J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology 1992, 102(03).
[11] Auttarat J, Phiriyangkul P, Utarabhand P. Characterization of vitellin from the ovaries of the banana shrimp Litopenaeus merguiensis [J]. Comparative biochemistry and physiology, Part B. Biochemistry & molecular biology 2006, 1(1).
[12] Chen YN., Kuo CM.. Purification and characterization of vitellin from the freshwater giantprawn, Macrobrachium rosenbergii [J]. Zoological Studies 1998, 2(2).
[13] 李建武, 余瑞元, 袁明秀. 生物化学实验原理和方法 [M]. 北京:北京大学出版社 2001.