通过MTT法研究了LaCl_3和GdCl_3对小鼠免疫细胞的作用.结果表明,GdCl_3在0.001～10 μmol/L浓度内均能促进小鼠脾细胞的增殖, 0.001～0.1 μmol/L的LaCl_3促进小鼠脾细胞的增殖,其他浓度没有影响;除0.1 μmol/L浓度外,其他测试浓度下的LaCl_3均促进小鼠T淋巴细胞的增殖;0.001 μmol/L的GdCl_3抑制小鼠T淋巴细胞的增殖,0.01～1 μmol/L时对小鼠T淋巴细胞的增殖没有影响,10 μmol/L时转而促进小鼠T淋巴细胞的增殖.浓度为0.1 μmol/L的LaCl_3和GdCl_3对小鼠B淋巴细胞的增殖有抑制作用,其他测试浓度下均促进小鼠B淋巴细胞的增殖.作用时间为4 h时,0.001 μmol/L的LaCl_3对NK细胞的活性没有影响,其他浓度下的LaCl_3均能提高NK细胞的活性,0.001～10 μmol/L的GdCl_3均能提高NK细胞的活性;作用时间为8 h时,0.001和0.01 μmol/L的LaCl_3显著降低NK细胞的活性,0.1和1 μmol/L的LaCl_3提高NK细胞的活性,当浓度为10 μmol/L时对NK细胞的活性没有影响,1 μmol/L的GdCl_3降低NK细胞的活性,其他测试浓度均能提高NK细胞的活性.这提示LaCl_3和GdCl_3对小鼠免疫细胞的影响模式与其作用浓度、作用时间以及稀土元素的种类都是密切相关的.
The effects of LaCl_3 and GdCl_3 on primary mouse immune cells in vitro were studied. The results indicated that GdCl_3(0.001-10 μmol/L) promoted the proliferation of the spleen cells. LaCl_3 had no effect on proliferation of the spleen cells at the concentrations of 1 and 10 μmol/L, but promoted the proliferation of the spleen cells at other concentrations. LaCl_3(0.001, 0.01, 1, and 10 μmol/L) promoted the proliferation of T lymphoid cells stimulated by concanavalin A (ConA). GdCl_3 inhibited the proliferation of T lymphoid cells stimulated by ConA at concentration of 0.001 μmol/L, had no effect on the proliferation of T lymphoid cells at the concentrations of 0.01, 0.1 and 1 μmol/L, but turned to promote the proliferation of T lymphoid cells at the concentration of 10 μmol/L. LaCl_3 and GdCl_3 inhibited the proliferation of B lymphoid cells stimulated by lipopolysaccharides (LPS) at the concentration of 0.1 μmol/L, but they promoted the proliferation of B lymphoid cells at other concentrations. For 4 h, LaCl_3 had no effect on NK cell activity at the concentration of 0.001 μmol/L, but promoted NK cell activity at other concentrations. GdCl_3(0.001, 0.01, 0.1,1, and 10 μmol/L) promoted NK cell activity. For 8 h, LaCl_3 inhibited NK cell activity at the concentrations of 0.001 and 0.01 μmol/L, promoted NK cell activity at the concentrations of 0.1 and 1 μmol/L, but had no effects on NK cell activity at the concentrations of 10 μmol/L. GdCl_3 inhibited NK cell activity at the concentration of 1 μmol/L, but it promoted NK cell activity at other concentrations. The results suggest that the effects of LaCl_3 and GdCl_3 on primary immune cells in vitro depend on time, concentration and their species.
[1] 李荣昌, 杨晓达, 王夔, 倪嘉缵. 我国稀土生物无机化学研究进展 [J]. 中国稀土学报 2004.doi:10.3321/j.issn:1000-4343.2004.01.001
[2] 马文强, 冯杰, Ma Wenqiang, Feng Jie. 稀土元素对动物免疫功能的影响 [J]. 饲料工业 2006.doi:10.3969/j.issn.1001-991X.2006.04.015
[3] 魏雪涛, 蒋建军, 尚兰琴, 贾风兰, 薛彬, 徐厚恩. 混合稀土对小鼠免疫功能的影响 [J]. 环境与职业医学 2002.doi:10.3969/j.issn.1006-3617.2002.03.059
[4] 涂序珉, 吕慧敏, 张翠兰, 齐雪松, 夏英, 张志兴, 董金婵. 稀土对受照射小鼠免疫功能的影响 [J]. 中华放射医学与防护杂志 2005.doi:10.3760/cma.j.issn.0254-5098.2005.03.008
[5] 申秀英, 许晓路, 章子贵, 巩菊芳, 赵建杰. 稀土元素铈和钐对大鼠血液学指标的影响 [J]. 营养学报 2004.doi:10.3321/j.issn:0512-7955.2004.06.024
[6] FATIMA R D, JOSEALEXANDRE M B, MARISTELA T. Discrimination between NK and LAK cytotoxic activities of murine spleen cell by MTT assay:differential inhibition by PGE2 and EDTA [J]. Journal of Immunological Methods 2000, 241.
[7] MOSMANN T. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays [J]. Journal of Immunological Methods 1983, 65.
[8] 王宗惠, 庞新民, 苏英. 稀土防癌实验及对NK 细胞活力影响的观察 [J]. 卫生毒理学杂志 1994, 8(03).
[9] 郭来有, 冯素芳, 王玉珍. 稀土作业对人体体液免疫功能的影响 [J]. 职业与健康 1997, 13(04).
