提出了一种新的谱图记录模式,以迁移时间t与分离电压V的乘积的负倒数(-(1/t·V))来代替原来的迁移时间t作为新谱图的横坐标,并在新坐标体系中选择合适的参照物谱峰,对变换后的电泳谱图实施平移操作.选择中药赤芍为分析对象考察了这种新变换在区带电泳和胶束电动色谱条件下对重现性的影响.实验表明对于不同电压下的电泳谱图,由处理前的迁移时间相对标准偏差(RSD)33%,降为处理后的(-(1/t·V))RSD 1.5%;对于平行实验间电泳谱图,由处理前的迁移时间RSD介于0.90%～1.39%, 降为处理后的(-(1/t·V))RSD介于0.17%～0.70%.这些结果表明,本文提出的谱图变换方式可显著提高谱图可视化比较的能力.
A new electropherogram mode was proposed by transforming time x-scale into minus reciprocal of the product of migration time and applied voltage. After a proper peak in the new electropherogram was assigned as the reference, each electropherogram was then moved along x-scale at a proper distance. The positive effect of new electropherogram on visual comparison was demonstrated by analyzing Radix paeoniae rubrain in capillary zone electrophoresis and mecellar electrokinetic chromatography. The relative standard deviation (RSD) in migration time was around 33% for each chosen peak when applied voltage varied from 15 kV to 30 kV, while the RSDs were dropped into the range from 0.17% to 0.70%. All results indicated that the proposed transformation method can significantly improve the visualization comparison of the electropherograms obtained at different voltages, in different sample matrices.
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