为了确定AM真菌孢子萌发和菌丝生长的最佳水平条件,采用L_(16)(4~5)多因子正交实验对影响网状球囊霉孢子萌发和菌丝生长的因素进行了优化选择.结果表明:优组合为A_4B2C_2D_3E_3,即pH 8,水琼脂质量浓度10 g/L,培养周期10 d,后熟条件20 d,培养温度28 ℃为最佳组合.同时发现在优组合下孢子不仅萌发得早,萌发率高,菌丝生长也很旺盛.
In order to determine the optimal level condition of spore germination and hyphal growth, several factors that influence spores germination and hyphal growth of arbuscular mycorrhizal fungi (AMF) were optimized by L_(16)(4~5) multifactor orthogonal experiment and the best level of spore germination was determined. The results showed that the best technical conditions were as following: pH 8, 10 g/L of water agar, 10 days of culture cycle, 20 days of aging storage, 28 ℃ of culture temperature. We found not only the spore germination was earlier and the germination rate of spores was higher, but also the hyphal growth was more stronger under the optimal condition.
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