为了降低昆虫分子系统学研究中的成本,提高资源利用率,本实验利用干制标本提取基因组DNA.通过对河北大学馆藏30年的漠甲亚科不同保存期干标本与液浸标本DNA的提取和特定基因片段PCR(聚合酶链式反应)扩增,获得一致效果. 通过细胞色素B (Cytb) 基因片段序列信息构建系统树并与传统分类学做比较,探讨了漠王族部分种类的系统进化关系,研究结果支持Reitter(1893)建立的 Platyopini和Pimeliini是2个独立族的观点,而不同意Beutle等(2005)将它们合并为1个族的观点. 研究结果为利用鞘翅目昆虫干标本进行分子系统学研究提供了借鉴.
In order to reduce insect molecular systematics research costs and improve resource utilization,dried specimens to extract genomic DNA were used.DNA was extracted from dried specimens and liquid dip samples of Pimeliinae kept in Museum of Hebei University for different storage life, which was used as template to amplify some specific gene fragments via PCR. No significant difference was noticed between those DNA from different samples. UPGMA phylogenetic tree was made up using the sequence information of Cytochrome B (Cytb) genetic fragments, and which was compared with traditional taxonomy to discuss the phylogenetic relationships of tribe Platyopini. Results support Reitter's opinion(2005) that Platyopini and Pimeliini belong to two tribes, negate Beutle's standpoint(1893) that belong to one tribe. This paper also provides experience for molecular systematic research of using dried coleoptera insect specimens.
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