用平衡透析和差示吸收光谱法详细研究了Pb2+与人血清白蛋白(HSA)的相互作用.平衡透析研究结果表明,Pb2+与HSA的相互作用明显受到缓冲溶液pH影响, 在pH 6.3和pH 5.4时, Pb2+在HSA上的强结合位点数分别是2.1个和1.1个,弱结合位点数分别为7.0个和2.4个,通过非线性最小二乘法拟合Bjerrum方程,首次报道了Pb2+-HSA体系的逐级稳定常数,Hill系数表明Pb2+与HSA的结合具有负协同效应.使用差示吸收光谱法研究物质的量比为1:1的Pb2+-HSA体系的电荷转移谱带,发现组氨酸咪唑基是Pb2+在白蛋白中的优先配位基团.进一步根据Zn2+与Pb2+竞争结合HSA上的强结合位点,推断Pb2+在HSA中优先结合位点是位点A.
[1] 吴根华, 汪春华, WU Gen-hua, WANG Chun-hua. 荧光法研究Pb2+与牛血清白蛋白的相互作用 [J]. 光谱学与光谱分析 2005.doi:10.3321/j.issn:1000-0593.2005.02.024
[2] 沈星灿, 边贺东, 涂楚桥, 张宏志, 梁宏, 周永洽, 申泮文, SHEN Xin-Can, BIAN He-Dong, TU Chu-Qiao, ZHANG Hong-Zhi, LIANG Hong, ZHOU Yong-Qia, SHEN Pan-Wen. Ni(Ⅱ)与HSA或BSA的结合平衡研究 [J]. 无机化学学报 2000.doi:10.3321/j.issn:1001-4861.2000.01.013
[3] 涂楚桥, 张宏志, 梁宏. Cd(Ⅱ)与HAS或BSA的结合平衡研究 [J]. 化学学报 2000.doi:10.3321/j.issn:0567-7351.2000.02.019
[4] 贺进田, 王改珍. 锌-牛血清白蛋白的电荷转移谱带研究 [J]. 光谱学与光谱分析 2001.doi:10.3321/j.issn:1000-0593.2001.05.005
[5] TERAYAMA K. Effects of lead on electrophoretic mobility, membrane sialic acid, deformability and survival of rat erythrocytes [J]. Indian Health 1993, 31.
[6] Erol Ayranci, Osman Duman. Binding of Lead Ion to Bovine Serum Albumin Studied by Ion Selective Electrode [J]. Protein and peptide letters 2004, 4(4).
[7] Sadler PJ., Viles JH.. H-1 AND CD-113 NMR INVESTIGATIONS OF CD2+ AND ZN2+ BINDING SITES ON SERUM ALBUMIN - COMPETITION WITH CA2+, NI2+, CU2+, AND ZN2+ [J]. Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics 1996, 15(15).
[8] Stewart AJ, Blindauer CA, Berezenko S, Sleep D, Sadler PJ. Interdomain zinc site on human albumin. [J]. Proceedings of the National Academy of Sciences of the United States of America 2003, 7(7).
