为阐明低氧胁迫对日本沼虾呼吸代谢和氧化代谢的影响,并初步探讨其作用机制及日本沼虾的抗氧化响应机制,将日本沼虾暴露于低氧((2±0.2) mg/L, 8 h)而后恢复常氧水平((7±0.2) mg/L, 2.5 h).结果显示:与对照组(保持常氧水平)相比,随着低氧暴露时间延长,日本沼虾肝胰腺和肌肉组织细胞色素氧化酶(CCO)和琥珀酸脱氢酶(SDH)活力显著下降(p＜0.05),延胡索酸还原酶(FRD)和乳酸脱氢酶(LDH)活力显著上升(p＜0.05);总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活力显著上升(p＜0.05),超氧化物歧化酶(SOD)活力显著下降(p＜0.05).恢复常氧阶段,CCO,SDH,FRD,LDH,CAT和SOD 活力逐渐恢复到正常水平,T-AOC在恢复常氧1 h时显著降低(p＜0.05),而后恢复到正常水平.低氧胁迫使得有氧代谢减弱,无氧代谢增强,以维持机体能量需求;T-AOC和CAT活力增加而SOD 活力降低,是日本沼虾适应低氧环境所采取的一种抗氧化策略.
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