对顺铂的主要代谢物[Pt(L-Met-S,N)2](Met:蛋氨酸)在Jurkat肿瘤细胞和绵羊红细胞内的跨膜吸收进行了研究,并以顺铂作对照.细胞内铂含量使用等离子体质谱(ICP-MS)测定.结果表明:2种细胞对[Pt(L-Met-S,N)2]的吸收曲线趋势一致,但吸收能力要远远低于对顺铂的吸收,这可能与[Pt(L-Met-S,N)2]的跨膜机制相关,同时进一步证实[Pt(L-Met-S,N)2]没有生物活性.
[1] 吴刚, 李荣昌, 乔秀文, 王坚成. 具有不同电荷和手性的Co(Ⅲ)配合物跨人红细胞膜的动力学研究 [J]. 高等学校化学学报 2001.doi:10.3321/j.issn:0251-0790.2001.07.001
[2] BECKER B F, DNHM J. Evidence for anionic cation transport of lithium, sodium and potassium across the human erythrocyte membrane induced by divalent anions [J]. Journal of Physiology 1978, 282.
[3] KARNISKI L P. Hg2+ and Cu+ are ionophores, mediating Cl-/OH-exchange in liposomes and rabbit renal brush border membranes [J]. Journal of Biological Chemistry 1992, 267(27).
[4] Kui Wang, Jingfen Lu, Ronchang Li. The events that occur when cisplatin encounters cells [J]. Coordination chemistry reviews 1996, 0(0).
[5] OHASHI K, KAJIYA K, INABA S. Copper(Ⅱ) protects yeast against the toxicity of cisplatin independently of the induction of metallothionein and the inhibition of platinum uptake [J]. Biochemical and Biophysical Research Communications 2003, 310.
[6] Zisowsky J, Koegel S, Leyers S, Devarakonda K, Kassack MU, Osmak M, Jaehde U. Relevance of drug uptake and efflux for cisplatin sensitivity of tumor cells. [J]. Biochemical Pharmacology 2007, 2(2).
[7] GATELY D P, HOWELL S B. Cellular accumulation of the anticancer agent cisplatin [J]. British Journal of Cancer 1993, 67.
[8] Ishida S, Lee J, Thiele DJ, Herskowitz I. Uptake of the anticancer drug cisplatin mediated by the copper transporter Ctr1 in yeast and mammals. [J]. Proceedings of the National Academy of Sciences of the United States of America 2002, 22(22).
[9] Elene Pereira-Maia, Arlette Garnier-Suillerot. Impaired hydrolysis of cisplatin derivatives to aquated species prevents energy-dependent uptake in GLC4 cells resistant to cisplatin [J]. Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry 2003, 6(6).
[10] STEWART D J. Mechanisms of resistance to cisplatin and carboplatin [J]. Critical Reviews in Oncology/Hematology 2007, 63.
[11] Zou J., Yang XD., An F., Wang K.. Relationship between cellular uptake rate and chemical behavior ofdiammine/diaminocyclohexane platinum (II) complexes with oxygen-ligatinganionic groups [J]. Journal of Inorganic Biochemistry: An Interdisciplinary Journal 1998, 3/4(3/4).
[12] Ghezzi A, Aceto M, Cassino C, Gabano E, Osella D. Uptake of antitumor platinum(II)-complexes by cancer cells, assayed by inductively coupled plasma mass spectrometry (ICP-MS) [J]. Journal of Inorganic Biochemistry: An Interdisciplinary Journal 2004, 1(1).
[13] RILEY B M, STERNSON L A, REPTA A J. Monitoring the reactions of cisplatin with nucleotides and methionine by reversed-phase high-performance liquid chromatography using cationic and anionic pairing ions [J]. Analytical Biochemistry 1982, 130.
[14] MURDOCH P del S, RANFORD J D, SADLER P J. Cis-trans isomerization of[bis(L-methioninato)platinum]:metabolite of the anticancer drug cisplatin [J]. Inorganic Chemistry 1993, 32.
[15] Haiying Wei, Qin Liu, Jun Lin, Pengju Jiang, Zijian Guo. Fast displacement of S, N-chelated L-methionine in platinum(II)complexes by biological thiols [J]. Inorganic Chemistry Communications 2004, 6(6).
[16] 刘琴. 铂类化合物与含硫、含硒生物分子的相互作用研究 [D]. 南京大学 2003.
[17] FREEMAN H C, GLOMB M L. Model compounds for metal-protein interaction:crystal structure of three platinum, (II) complexes of L-and DL-methionine and glycyl-L-methionine [J]. Journal of the Chemical Society Dalton Transactions 1970.
[18] Christian K. Riener, Gerald Kada, Hermann J. Gruber. Quick measurement of protein sulfhydryls with Ellman's reagent and with 4, 4'-dithiodipyridine [J]. Analytical and bioanalytical chemistry 2002, 4/5(4/5).
