利用噬菌体展示技术筛选只结合1种对应体的BINOL抗体,建立人类单链抗体(scFv)噬菌体文库--Griffin.1文库,以固相化抗原淘筛抗体库,ELISA鉴定噬菌体抗体.从经过4轮富集的次级抗体库中挑选到噬菌体克隆,用该克隆制备的单链抗体阳性克隆,经ELISA证实具有良好的抗原特异性.从人类scFv噬菌体文库中获得抗BINOL的特异性抗体.
[1] WILMINGTON. Protein design and phage display ronald H [J]. Chemical Reviews 2001, 101.
[2] WINTER G, GRIFFITHS AD, HAWKINS RE. Making antibodies by phage display technology [J]. Annual Review of Immunology 1994, 12.
[3] EMMANUELLE BONNIN, A NADèGE GRUEL. Generation of functional scFv intrabodies for triggering anti-tumor immunity [J]. Methods:A Companion to Methods in Enzymology 2004, 34.
[4] SMITH G P. Filamentous fusion phage:novel expression vectors that display cloned antigens on the virion surface [J]. Science 1985, 228.
[5] Wenjun Tang, Xumu Zhang. New Chiral Phosphorus Ligands for Enantioselective Hydrogenation [J]. Chemical Reviews 2003, 8(8).
[6] MCCAFFERTY J, GRIFFITHS AD, WINTER G. Phage antibodies:filamentous phage displaying antibody variable domains [J]. Nature 1990, 348.
[7] GRIFFITHS A D, MALMQVIST M, MARKS JD. Human anti-self antibodies with high specificity from phage display libraries [J]. EMBO Journal 1993, 12.
[8] Hoogenboom HR. Designing and optimizing library selection strategies for generating high-affinity antibodies. [J]. Trends in Biotechnology 1997, 2(2).
[9] HOOGENBOOM H R, GRIFFITHS AD, JOHNSON KS. Multi-subunit proteins on the surface of filamentous phage:methodologies for displaying antibody (Fab) heavy and light chains [J]. Nucleic Acids Research 1991, 19.
[10] CHANG C N, LANDOLFI NF, QUEEN C. Expression of antibody Fab domains on bacteriophage surfaces [J]. Journal of Immunology 1991, 147.
[11] BARBAS CF, KANG AS, LERNER RA. Assembly of combinatorial antibody libraries on phage surfaces:the gene Ⅲsite [J]. Proceedings of the National Academy of Sciences(USA) 1991, 88.
[12] RAKONIJC J, JOVANOVIC G, MODEL P. Filamentous phage infectionmediated gene expression:construction and propagation of the gill deletion mutant helper phage R408d3 [J]. Gene 1997, 198.
[13] BARBAS C F, WAGNER J. Synthetic human antibodies:selecting and solving functional proteins [J]. METHODS-A COMPANION TO METHODS IN ENZYMOLOGY 1995, 8.
