实验将3' 端修饰巯基的寡聚核苷酸标记在纳米银粒子上,并基于银纳米粒子溶解后产生的 Ag+对K2S2O8-luminol 化学发光体系的催化作用,对标记寡聚核苷酸的银纳米粒子进行了定量检测.探讨了各种实验条件对该体系的影响并建立了最佳分析条件.其线性范围为5×10-9～1×10-6 g/mL,检出限(3σ)为2.0×10-9 g/mL.该方法操作简便,灵敏度高,为进一步的DNA杂交分析提供了一种新的检测手段.
[1] 李正平, 陈晓, LI Zheng-ping, CHEN Xiao. 过氧化草酸酯化学发光检测DNA荧光探针 [J]. 河北大学学报（自然科学版） 2006.doi:10.3969/j.issn.1000-1565.2006.02.014
[2] CHRISTA C M. Protein assay sensitive at nanogram levels [J]. Analytical Biochemistry 1987, 160.
[3] Thanh NTK., Rosenzweig Z.. Development of an aggregation-based immunoassay for anti-protein A using gold nanoparticles [J]. Analytical chemistry 2002, 7(7).
[4] Zhang C., Zhang ZY., Yu BB., Shi JJ., Zhang XR.. Application of the biological conjugate between antibody and colloid Au nanoparticles as analyte to inductively coupled plasma mass spectrometry [J]. Analytical chemistry 2002, 1(1).
[5] T.Andrew Taton, Gang Lu, Chad A.Mirkin. Two-Color Labeling of Oligonucleotide Arrays via Size-Selective Scattering of Nanoparticle Probes [J]. Journal of the American Chemical Society 2001, 21(21).
[6] 张新荣, 章竹君. 鲁米诺-K2S2O8体系中金属离子化学发光行为的研究 [J]. 化学学报 1987, 45.
