利用双向电泳技术比较正常和性早熟河蟹视神经节蛋白的表达差异,旨在探索蛋白质组学技术在甲壳动物中尤其是河蟹的性早熟研究当中的应用.结果发现,明显差异的蛋白点共24个,为以后通过蛋白质组学手段筛选出真正影响性早熟发生的蛋白质或酶奠定了基础.
[1] 柳志会, 孙福新. 中华绒螯蟹池塘养殖性早熟产生原因的初步分析与其预防 [J]. 海水养殖 1992.
[2] 金刚. 早熟河蟹的养殖生态学及渔业价值评估 [J]. 水生生物学报 1998, 22(02).
[3] KELLER R. Crastacean neuropeptides:strtlctures, functions and comparative aspeets [J]. Experimential 1992, 48(05).
[4] 李展虹, 王成辉, 李思发. 中华绒螯蟹性早熟的早期判别 [J]. 水产科技情报 1998, 25(02).
[5] WlLKINS M R, SANCHEZ J C, GOOLEY A A. Proess with proteome projects:why all proteins expressed by a genome should be identified and how to do it [J]. Biotechnology and Genetic Engineering Reviews 1993, 13.
[6] Choe LH., Lee KH.. A comparison of three commercially available isoelectric focusing units for proteome analysis: The Multiphor, the IPGphor and the Protean IEF cell [J]. Electrophoresis: The Official Journal of the International Electrophoresis Society 2000, 5(5).