对14株不同宿主来源的Frankia纯培养菌株进行了形态观察和固氮活性测定.结果表明,14株供试菌株都具有分枝菌丝、泡囊或孢囊等形态特征,其中大花胡颓子分离菌49121有特殊的串珠状菌丝链结构;所测菌株全部具有固氮活性,其中菌株49136固氮活性最高,乙炔还原活性达到78.5μmol/(mg·h);另外,菌株的固氮活性与泡囊数量有一定的正相关性.
[1] 阮继生. 放线菌分类基础 [M]. 北京:科学出版社 1997.
[2] CALLAHAM D, TORREY J G, TREDICI P D. Isolation and cultivation in vitro of the Actinomycete causing root nodulation in Componia [J]. Science 1978, 199.
[3] MURRY M A, FOUTAINE M S, TORREY J G. Growth kinetics and nitrogenase induction in Frankia sp.HFPArI3 grown in batch culture [J]. Plant and Soil 1984, 78.
[4] BAKER D, DANELL K H. Effects of oxygen and chloramphenicol on Frankia nitrogenase activity [J]. Archives of Microbiology 1986, 144.
[5] TJEPKEMA J D, TORREY J G. Factor affecting vesicle formation and acetylene reduction (nitrongenase activity) in Frankia sp.CpI1 [J]. Canadian Journal of Microbiology 1983, 27.
[6] BRADFORD M M. A rapid and sensitive method for the quantification of microgram quantities of protein principle of protein dye binding [J]. Analytical Biochemistry 1976, 72.
