根据过氧化草酸酯与双氧水作用产生的高能中间体激发荧光物质发光的原理,研究了利用过氧化草酸酯TCPO-H2O2化学发光体系测定Cy5标记的DNA探针的新方法,探讨了溶剂、酸度、催化剂及发光溶液浓度对化学发光信号的影响,确立了最佳分析条件.其线性范围为8.4×10-9～2.8×10-6mo1/L,检出限(3σ)为2.8×10-9 mol/L.据此建立了操作简便、灵敏度高的测定Cy5标记的DNA探针的新方法.
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