研究了AgNO3和低温处理对小麦悬浮细胞谷胱甘肽转移酶(GST)及谷胱甘肽还原酶(GR)酶活性的影响.结果表明,AgNO3和低温处理对小麦细胞再生的影响因处理时间的不同而有差异,在处理的4 d内低温处理使再生率明显增加,AgNO3处理2 d使细胞再生率显著增高,但处理4 d则对细胞再生无明显影响.低温处理使细胞蛋白质含量明显升高,AgNO3处理对小麦细胞蛋白质含量无明显影响.小麦细胞GST活性随处理时间的延长而迅速降低,AgNO3处理对GST活性无明显影响,但低温处理明显延缓GST活性降低.AgNO3和低温处理都使小麦细胞GR酶活性明显降低.AgNO3处理2 d和低温处理对细胞谷胱甘肽(GSH)含量无明显影响,但在处理的第4 d AgNO3使细胞GSH含量明显降低.
[1] 廖祥儒, 方怡震, 朱新产. 植物谷胱甘肽转移酶 [J]. 生命的化学 1996, 16(06).
[2] Marrs KA. THE FUNCTIONS AND REGULATION OF GLUTATHIONE S-TRANSFERASES IN PLANTS [Review] [J]. Annual Review of Plant Physiology & Plant Molecular Biology 1996, 0(0).
[3] 陈英, 李向辉, 孙勇如. 植物细胞培养与遗传操作 [M]. 长沙:湖南科学技术出版社 1992.
[4] HENR Y Y, DE BUYSER J. Float culture of wheat anther [J]. Theoretical and Applied Genetics 1981, 60.
[5] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principles of dye-binding [J]. Analytical Biochemistry 1976, 72.
[6] TARADA T, MAEDA H, OKAMOTO K. Modulation of glutathione S- transferase activity by a thiol/disulfide exchange reaction and enrolment of thioltransferase [J]. Archives of Biochemistry and Biophysics 1993, 300.
[7] Rao MV., Ormrod DP., Paliyath C.. ULTRAVIOLET-B- AND OZONE-INDUCED BIOCHEMICAL CHANGES IN ANTIOXIDANT ENZYMES OF ARABIDOPSIS THALIANA [J]. Plant physiology 1996, 1(1).
[8] Ellman G L. A colorimetric method for determining low concentrations of mercaptans [J]. Archives of Biochemistry and Biophysics 1958, 74.
[9] DROOG F N J, HOOYKAAS PJ J, VANDER ZAAL B J. 2 4-Dichlorophenoxyacetic acid and related chlorinated compounds inhibit two auxin-regulated type-Ⅲ tobacco glutathione S-transferase [J]. Plant Physiology 1995, 107.
