成熟中国对虾(Penaeus chinensis)卵巢中卵黄蛋白的纯化

柳峰松; 武金霞; 谢松; 房宝玲; 黄增瑞; 吴琛

doi:10.3969/j.issn.1000-1565.2001.04.017

河北大学学报(自然科学版) >

2001 , Vol. 21 >Issue 4: 406 - 410

DOI: https://doi.org/10.3969/j.issn.1000-1565.2001.04.017

成熟中国对虾(Penaeus chinensis)卵巢中卵黄蛋白的纯化

柳峰松 ,
武金霞 ,
谢松 ,
房宝玲 ,
黄增瑞 ,
吴琛

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1.河北大学,生命科学学院,河北,保定,071002； 2.邯郸教育学院,教务处,河北,邯郸,056000

网络出版日期: 2001-10-25

基金资助

河北省自然科学基金

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Purification of Vitellin in Mature Ovary of Prawn,Penaeus chinensis

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Online published: 2001-10-25

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摘要

用层析(SephadexG-150, DEAE-Cellulose-52)和电泳洗脱2种方法分别从成熟中国对虾卵巢中纯化得到了一种卵黄蛋白,聚丙烯酰胺凝胶电泳(PAGE)检测表明,卵黄蛋白出现在SephadexG-150的第1个洗脱峰和DEAE-Cellulose-52的0.4 mol/L和0.5 mol/L NaCl洗脱峰处.比较了这2种方法的优缺点:层析法可用于蛋白的大量制备,电泳洗脱能够得到高纯度的蛋白.并对与其结合的类胡萝卜素的稳定性进行了讨论.

关键词： 中国对虾; 卵黄蛋白; 纯化; 层析; 电泳洗脱

本文引用格式

柳峰松 , 武金霞 , 谢松 , 房宝玲 , 黄增瑞 , 吴琛 . 成熟中国对虾(Penaeus chinensis)卵巢中卵黄蛋白的纯化[J]. 河北大学学报(自然科学版), 2001 , 21(4) : 406 -410 . DOI: 10.3969/j.issn.1000-1565.2001.04.017

参考文献

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