对虾一种杆状病毒的分离纯化及超微结构

  • 孙磊 ,
  • 王维娜 ,
  • 郭明申 ,
  • 苏磊 ,
  • 李凤超 ,
  • 王军霞 ,
  • 张辉 ,
  • 王安利
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  • 河北大学,生命科学学院,河北,保定,071002

网络出版日期: 2004-01-25

基金资助

河北省科技成果推广计划,河北省农业综合开发资助项目

Purification and Ultrastructure of a Baculovirus in Penaeid Shrimps

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Online published: 2004-01-25

摘要

从市售的甲壳上带有白斑的冷冻斑节对虾(Penaeus monod on)中提取到一种杆状病毒,并用它感染了活体的中国对虾(Penaeus chinensis) 幼虾和日本对虾(Penaeus japonicus).这2种对虾均发病死亡.从死亡的对虾中再次提纯出此杆状病毒.此种杆状病毒的核酸为DNA,衣壳的主要结构多肽的相对分子质量为49 .8 kDa.通过对此杆状病毒悬液负染片的电镜观察,对其超微结构进行了研究并提出了一种对虾杆状病毒衣壳超微结构的模式图.

本文引用格式

孙磊 , 王维娜 , 郭明申 , 苏磊 , 李凤超 , 王军霞 , 张辉 , 王安利 . 对虾一种杆状病毒的分离纯化及超微结构[J]. 河北大学学报(自然科学版), 2004 , 24(1) : 76 -82 . DOI: 10.3969/j.issn.1000-1565.2004.01.018

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