中国人神经珠蛋白(NGB)基因克隆与序列分析

  • 牛建章 ,
  • 田侠 ,
  • 赵慧 ,
  • 叶步青 ,
  • 廖祥儒 ,
  • 赵晓瑜
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  • 1.河北大学,生命科学学院,河北,保定,071002; 2.中国科学院,上海生物工程研究所,上海,200000

网络出版日期: 2005-07-25

基金资助

河北省科技厅科研项目

Cloning and Sequencing of Chinese Neuroglobin(NGB)Gene

  • NIU Jian-Zhang ,
  • Tian Xia ,
  • ZHAO Hui ,
  • YE Bu-qing ,
  • LIAO Xiang-ru ,
  • ZHAO Xiao-Yu
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Online published: 2005-07-25

摘要

应用RT-PCR方法从中国人胎脑组织中扩增出神经珠蛋白(NGB)特异性编码基因片段,并将其克隆到pMD18-TVector中,构建了pMD18-T-hNGB克隆质粒,然后进行测序分析并与国外报道的NGB序列相比较.结果表明,本文获得的中国人NGB编码基因序列与国外序列的同源性为98%.

本文引用格式

牛建章 , 田侠 , 赵慧 , 叶步青 , 廖祥儒 , 赵晓瑜 . 中国人神经珠蛋白(NGB)基因克隆与序列分析[J]. 河北大学学报(自然科学版), 2005 , 25(4) : 412 -417 . DOI: 10.3969/j.issn.1000-1565.2005.04.019

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