采用化学交联法,以1-(3-二甲氨基丙基)-3-乙基碳二亚胺(EDC)为脱水剂、4-二甲基氨基吡啶(DMAP)为催化剂,将透明质酸(Hyaluronic acid,HA)接枝到普鲁兰(Pullulan,Pu)糖长链上,制备透明质酸-普鲁兰糖(HA-Pu)新型材料.傅里叶变换红外和氢核磁表征显示,成功合成HA-Pu材料,并用1H NMR法确定HA-Pu的透明质酸取代度.合成新材料冻干后可压制获得HA-Pu膜,扫描电镜下观察,HA-Pu膜为层状结构,具备很多微小孔洞.体外酶降解实验表明,新型HA-Pu膜较透明质酸具有更好的抵抗酶降解性能.HA-Pu新材料合成有望拓展透明质酸在医药领域的应用.
A series of hyaluronic acid grafted pullulan(HA-Pu)were prepared by esterification reaction,using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide(EDC)as dehydrating agent and 4-dimethylaminopyridine(DMAP)as catalyst.The structure of HA-Pu was confirmed by the Fourier transform infrared spectrum(FTIR)and proton nuclear magnetic resonance(1H NMR),and the degree of substitution(DS)of hyaluronic acid moiety was determined by 1H NMR.The freeze-dried HA-Pu materials could be compressed to obtain a HA-Pu film.The HA-Pu film was observed by scanning electron microscopy(SEM),which obtained the layered microstructure with a lot of small holes.Compared with hyaluronic acid,the HA-Pu materials have better ability to resist enzyme degradation in vitro.Therefore,HA-Pu should be a promising novel material to expand the application of hyaluronic acid in pharmaceatics.
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