苯酚污染对蚯蚓抗氧化酶系活性及同工酶组分的影响

武金霞; 范君姣; 赵敬敬; 张贺迎

doi:10.3969/j.issn.1000-1565.2017.02.008

河北大学学报(自然科学版) >

2017 , Vol. 37 >Issue 2: 155 - 160

DOI: https://doi.org/10.3969/j.issn.1000-1565.2017.02.008

苯酚污染对蚯蚓抗氧化酶系活性及同工酶组分的影响

武金霞 ,
范君姣 ,
赵敬敬 ,
张贺迎

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武金霞(1966—),女,河北保定人,河北大学教授,博士,主要从事生化药物及基因工程药物研究. E-mail:wjx6@163.com

收稿日期: 2016-06-30

网络出版日期: 2017-03-25

基金资助

河北省生物工程重点学科资助项目(1050-5030023)

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Effect of phenol on antioxidant enzymes activity and isozymes composition of Eisenia foetida

WU Jinxia ,
FAN Junjiao ,
ZHAO Jingjing ,
ZHANG Heying

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1.College of Life Sciences, Hebei University, Baoding 071002, China; 2.Research Center for Biotechnology, Hebei University, Baoding 071002, China

Received date: 2016-06-30

Online published: 2017-03-25

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摘要

采用滤纸接触法,研究了不同质量浓度、不同时间苯酚污染胁迫对赤子爱胜蚓(Eisenia foetida)超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及同工酶组分的影响,为研究酚类化合物对土壤污染的生物学诊断和风险评价提供科学依据.结果表明,苯酚对蚯蚓SOD、POD和CAT活性及同工酶组分具有显著的影响,不同质量浓度苯酚可显著提高蚯蚓CAT活力,改变蚯蚓SOD、POD同工酶组分,低质量浓度苯酚可提高蚯蚓SOD和POD活力,高质量浓度则会降低其活力.

关键词： 苯酚; 蚯蚓; 超氧化物歧化酶(SOD); 过氧化物酶(POD); 过氧化氢酶(CAT)

本文引用格式

武金霞 , 范君姣 , 赵敬敬 , 张贺迎 . 苯酚污染对蚯蚓抗氧化酶系活性及同工酶组分的影响[J]. 河北大学学报(自然科学版), 2017 , 37(2) : 155 -160 . DOI: 10.3969/j.issn.1000-1565.2017.02.008

Abstract

The filter paper contacting test was conducted to detect the activity and isozymes composition of SOD,POD and CAT of Eisenia foetida exposured with phenol,to provide scientific basis for biological diagnosis and risk assessment of soil pollution by phenol compounds.The results suggested that the CAT activity was activated markedly at different concentrations;the SOD and POD isozymes composition was changed; the SOD and POD activity were induced prominently at low concentration but inhibited at high concentration.

Key words： phenol; earthworm; SOD; POD; CAT

参考文献

[1] LI Z C,TANG W R,ZHU J K,et al.Simultaneous determination of phenolic pollutants in waste water using CE-AD[J].Journal of East China Normal University(Natural Science),2013(1):128-138.DOI:10.3969/j.issn.1000-5641.2013.01.016.
[2] 郑龙珍,董泽民,熊乐艳,等.WC/TiO₂纳米复合界面光催化剂制备及其光催化降解酚类污染物研究[J].环境科学学报,2014,34(11):2806-2814.DOI:10.13671/j.hjkxxb.2014.0596. ZHENG L Z,DONG Z M,XIONG L Y,et al.Preparation of WC/TiO₂ nanocomposite interfacial photocatalyst and its photocatalytic degradation of phenol pollutant[J].Acta Scientiae Circumstantiae,2014,34(11):2806-2814.DOI:10.13671/j.hjkxxb.2014.0596.
[3] 殷焕顺.纳米材料修饰电极对土壤及水中酚类污染物的检测研究[D].泰安:山东农业大学,2012.DOI:10.7666/d.y2117170. YIN H S.Determination of phenolic in soil and water samples based on nanomaterials modified electrodes[D].Taian:Shandong Agricultural University,2012.DOI:10.7666/d.y2117170.
[4] 胡婷,谷洁,甄丽莎,等.石油污染土壤中苯酚降解菌ad049的鉴定及降解特性[J].生态学报,2014,34(5):1140-1148.DOI:10.5846/stxb201306091528. HU T,GU J,ZHEN L S,et al.Identification and characteristics of phenol degrading bacteria ad049 screened from oil contaminated soil[J].Acta Ecologica Sinica,2014,34(5):1140-1148.DOI:10.5846/stxb201306091528.
[5] MOYERS B,WU J S.Removal of organic precursors by permanganate oxidation and alum coagulation[J].Water Research,1985,19(3):309-314.DOI:10.1016/0043-1354(85)90090-9.
[6] 张菊萍,陆少鸣,徐姮,等.臭氧-生物活性炭净水工艺处理苯酚污染的应急效果及影响因素分析[J].环境工程学报,2015,9(6):2745-2749. ZHANG J P,LU S M,XU H,et al.Phenol pollution emergency treatment and influencing factors of O₃-BAC process[J].Chinese Journal of Environmental Engineering,2015,9(6):2745-2749.
[7] 周垂帆,王玉军,俞元春,等.铜和草甘膦对蚯蚓的毒性效应研究[J].中国生态农业学报,2012,20(8):1077-1082.DOI:10.3724/SP.J.1011.2012.01077. ZHOU C F,WANG Y J,YU Y C,et al.Cu and glyphosate toxicity to earthworm(Eisenia fetida)[J].Chinese Journal of Eco-Agriculture,2012,20(8):1077-1082.DOI:10.3724/SP.J.1011.2012.01077.
[8] ACHAZI R K,FLENNER C,LIVINGSTONE D R,et al.Cytochrome P450 and dependent activities in unexposed and PAH-exposed terrestrial annelids[J].Comparative Biochemistry and Physiology,1998,121(1):339-350.DOI:10.1016/S0742-8413(98)10055-5.
[9] 孙仕仙,陶瑞,张庆蛟,等.乐果和杀虫双污染对蚯蚓体内蛋白质含量的影响[J].江西农业大学学报,2012,34(2):298-303.DOI:10.3969/j.issn.1000-2286.2012.02.018. SUN S X,TAO R,ZHANG Q J,et al.Effects of dimethoate and dimehypo pollution on protein content of earthworm[J].Acta Agriculturae Universitatis Jiangxiensis,2012,34(2):298-303.DOI:10.3969/j.issn.1000-2286.2012.02.018.
[10] LUO Y,ZANG Y,ZHONG Y,et al.Toxicological study of two novel pesticides on earthworm(Eisenia foetida)[J].Chemosphere,1999,39(13):2347-2356.DOI:10.1016/S0045-6535(99)00142-3.
[11] LIN D S,XIE X J,ZHOU Q X,et al.Biochemical and genotoxic effect of triclosan on earthworms(Eisenia fetida)using contact and soil tests[J].Environ Toxicol,2012,27:385-392.DOI:10.1002/tox.20651.
[12] GUO Y Y,LIU T,ZHANG J,et al.Biochemical and genetic toxicity of the ionic liquid 1-octyl-3-methylimidazolium chloride on earthworms(Eisenia fetida)[J].Environmental Toxicology and Chemistry,2016,35(2):411-418.DOI:10.1002/etc.3198.
[13] JEYANTHI V,PAUL J A J,SELVI B K,et al.Comparative study of biochemical responses in three species of earthworms exposed to pesticide and metal contaminated soil[J].Environ Process,2016,3:167-178.DOI:10.1007/s40710-016-0131-9.
[14] FENG L,ZHANG L,ZHANG Y N,et al.Inhibition and recovery of biomarkers of earthworm(Eisenia fetida)after exposure to thiacloprid[J].Environ Sci Pollut Res,2015,22:9475-9482.DOI:10.1007/s11356-015-4122-6.
[15] DUAN X C,XU L,SONG J,et al.Effects of benzo[a] pyrene on growth,the antioxidant system,and DNA damage in earthworms(Eisenia fetida)in 2 different soil types under laboratory conditions[J].Environmental Toxicology and Chemistry,2015,34(2):283-290.DOI:10.1002/etc.2785.
[16] MA T T,CHEN L K,WU L H,et al.Oxidative stress,cytotoxicity and genotoxicity in earthworm Eisenia fetida at different di-n-butyl phthalate exposure levels[J].PLoS ONE,2016,11(3):1-11.DOI:10.1371/journal.pone.0151128.
[17] ZHANG W,LIU K,LI J,et al.Uptake and depuration kinetics of lead(Pb)and biomarker responses in the earthworm Eisenia fetida after simultaneous exposure to decabromodiphenyl ether(BDE209)[J].Ecotoxicology and Environmental Safety,2015,113:45-51.DOI:10.1016/j.ecoenv.2014.11.014.
[18] 潘书娟.苯酚和汞污染对蚯蚓抗氧化酶和金属硫蛋白基因表达的影响[D].保定:河北大学,2011. PAN S J.Effect of phenol and hydrargrum ion on the antioxide enzymes and gene expression of metallothionein in Eisenia foetida earthworm[D].Baoding:Hebei University,2011.
[19] MARKLUND S,MARKLUND G.Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase[J].European Journal of Biochemistry,1974,47:469-479.DOI:10.1111/j.1432-1033.1974.tb03714.x.
[20] KOCHBA J,LAVEE S,SPIEGEL-ROY P.Differences in peroxidase activity and isoenzymes in embryogenic and nonembryogenic “Shamouti” orange ovular callus lines[J].Plant Cell Physiol,1977,18:463-467.
[21] SONG Y,ZHU L S,XIE H,et al.DNA damage and effects on antioxidative enzymes in earthworm(Eisenia fetida)induced by atrazine[J].Soil Biology and Biochemistry,2009,41(5):905-909.DOI:10.1016/j.soilbio.2008.09.009.
[22] 张丽娜.Fe²⁺、Mn²⁺胁迫对西洋参生长的影响[D].北京:中国农业科学院,2013. ZHANG L N.Effects of Fe²⁺ and Mn²⁺ stress on the growth of Panax quinquefolium L[D].Beijing:Chinese Academy of Agricultural Sciences,2013.
[23] 王兰,李涌泉,闫博,等.Cd对河南华溪蟹(Sinopotamon henanense)主要组织器官SOD和POD同工酶的影响[J].应用与环境生物学报,2007,13(6):823-829.DOI:10.3321/j.issn:1006-687x.2007.06.015. WANG L,LI Y Q,YAN B,et al.Effect of Cadmium on SOD and POD isozyme in tissues of fresh water crab(Sinopotamon henanense)[J].Chin J Appl Environ Biol,2007,13(6):823-829.DOI:10.3321/j.issn:1006-687x.2007.06.015.
[24] 武金霞,张贺迎,张瑞英,等.生物化学实验原理与技术[M].保定:河北大学出版社,2005:78-80.
[25] 曹佳,刁小平,胡继业,等.异噁草酮对蚯蚓抗氧化酶活性剂DNA损伤的研究[J].农业环境科学学报,2013,32(5):925-931.DOI:10.11654/jaes.2013.05.007. CAO J,DIAO X P,HU J Y,et al.Effects of clomazone on antioxidative enzymes activity and DNA damage of earthworm(Eisenia foetida)[J].Journal of Agro-Environment Science,2013,32(5):925-931.DOI:10.11654/jaes.2013.05.007.
[26] LIU S,ZHOU Q,CHEN C.Antioxidant enzyme activities and lipid peroxidation in erathworm(Eisena fetida)exposedto1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl-cylopenta-γ-2-benzopyran[J].Environmental Toxicology,2012,27(7):472-479.DOI:10.1002/tox.20661.

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