以异硫氰酸荧光素(FITC)为荧光分子,二氧化硅为生物相容性载体制备荧光纳米硅球.为增强硅球的稳定性,引入γ-氨丙基三乙氧基硅烷(APTES)做桥梁分子,由于FITC、SiO2与APTES连接顺序的非单一性,分别制备了表面连接型和核-壳型键合FITC的荧光硅球,并对其形貌和光漂白性进行了比较.实验结果表明,核-壳型和表面连接型荧光硅球形貌相似粒径接近,但表面连接型光漂白性严重.进而,对核-壳型中典型的包硅方法——Stöber法和反相微乳液法进行比较,结果表明,反相微乳液法制备的核-壳型键合FITC的荧光硅球荧光信号更强、粒径更小且分散性良好,同时在7 h内荧光染料泄漏程度小于8%,30 min内荧光强度仅降低2.2%,具备低染料泄露、光稳定性好等优点.
Silica fluorescent nanoparticles were prepared with fluorescein isothiocyanate(FITC)as the fluorescent molecule and silica as the biocompatible carrier.To enhance the stability of the microspheres,the bridge molecule(γ-aminopropyl triethoxysilane,APTES)was introduced.Owing to the non-unity of the connection sequences of FITC,SiO2 with APTES,two types(surface connection type and core-shell type)of FITC bonded fluorescent silica nanospheres were prepared and compared in their morphologies and photobleachings.The results show that both types had similar morphology,but the surface connection type was optically bleached seriously.Furthermore,two typical methods of packaging silicon(Stöber and reverse microemulsion)were compared.Core-shell FITC bonded fluorescent silica nanospheres preparaed by reverse microemulsion method showed stronger fluorescence signal,and had smaller particle size and better dispersibility.In addition the value of fluorescent dye leak was less than 8% within 7 h in the dye leakage experiment and the fluorescence intensity decreased only 2.2% within 30 min in light stability experiment,which showed the low dye leakage degree and good light stability properties.-
[1] 徐庆杰.功能性微球的可控制备与表征[D].无锡:江南大学,2013. XU Q J.The controllable preparation and characterization of functional microspheres[D].Wuxi:Jiangnan University,2013.
[2] 杜磊,刘烈雄,曹元成.基于光谱自编码微球的多样品酶联免疫检测混合筛选[J].精细化工,2004,21(9):667-670.DOI:10.13550/j.jxhg.2004.09.008. DU L,LIU L X,CAO Y C.Mixed screening of multi-samples in ELISA based on optical self-encoding microbeads[J].Fine Chemicals,2004,21(9):667-670.DOI:10.13550/j.jxhg.2004.09.008.
[3] 汪地强,刘白玲,胡杰,等.荧光微球的制备及应用[J].高分子材料科学与工程,2004,20(4):42-45.DOI:10.3321/j.issn:1000-7555.2004.04.010. WANG D Q,LIU B L,HU J,et al.The preparations and applications of fluorescent microsphere[J] ,Ploymer Materials Science and Engineering,2004,20(4):42-45.DOI:10.3321/j.issn:1000-7555.2004.04.010.
[4] 张荣荣,徐自立.荧光微球的制备技术及其应用进展[J].高分子通报,2009,1:63-70.DOI:10.14028/j.cnki.1003-3726.2009.01.005. ZHANG R R,XU Z L.Development of preparations and applications of fluorescent microsphere[J].Polymer Bulletin.2009,1:63-70.DOI:10.14028/j.cnki.1003-3726.2009.01.005.
[5] 陈中举,张燕玲,黄金瑛.荧光标记生物大分子及其应用[J].国外医学生物医学工程分册,2004,27(6):348-352.DOI:10.3760/cma.j.issn.1673-4181.2004.06.007. CHEN Z J,ZHANG Y L,HUANG J Y.The labeling of biological big molecule with fluorescence dye and its applications[J] ,Biomedical Engineering Foreign Medical Sciences,2004,27(6):348-352.DOI:10.3760/cma.j.issn.1673-4181.2004.06.007.
[6] ZHANG Q,WANG X L,LI P Z,et al.Biocompatible,uniform,and redispersible mesoporous silica nanoparticles for cancer-targeted drug delivery in vivo[J].Advanced Functional Materials,2014,24:2450-2461.DOI:10.1002/adfm.201302988.
[7] SCHULZ A,MCDONAGH C.Intracellular sensing and cell diagnostics using fluorescent silica nanoparticles[J].Soft Matter,2012,8(9):2579-2585.DOI:10.1039/c2sm06862a.
[8] 谢春娟,尹东光,李剑,等.新型FITC掺杂的二氧化硅荧光纳米粒子的合成及其应用于pH传感的研究[J].分析化学,2010,38(4):488-492.DOI:10.3724/SP.J.1096.2010.00488. XIE C J,YIN D G,LI J,et al.Preparation of a novel type of fluorescein isothiocyanate doped fluorescent silica nanoparticles and its application as pH probe[J].Chinese Journal of Analytical Chemistry,2010,38(4):488-492.DOI:10.3724/SP.J.1096.2010.00488.
[9] HAN Y,JIANG J,LEE S S,et al.Reverse microemulsion-mediated synthesis of silica-coated gold and silver nanoparticles[J].Langmuir,2008,24(11):5842-5848.DOI:10.1021/la703440p.
[10] SANTRA S,LIESENFELD B,BERTOLINO C,et al.Fluorescence lifetime measurements to determine the core-shell nanostructure of FITC-doped silica nanoparticles:An optical approach to evaluate nanoparticle photostability[J].Journal of Luminescence,2006,117:75-82.DOI:10.1016/j.jlumin.2005.04.008.
[11] BUMS A,SENGUPTA P,ZEDAYKO T,et al.Core/shell fluorescent silica nanoparticles for chemical sensing:towards single-particle laboratories[J].Small,2006,2(6):723-726.DOI:10.1002/smll.200600017.
[12] ZHANG X,SONG C,CHEN L,et al.A novel immunoassay for residual bovine serum albumin(BSA)in vaccines using laser-induced fluorescence millimeter sensor array detection platform[J].Biosensors and Bioelectronics,2011,26:3958-3961.DOI:10.1016/j.bios.2011.02.050.
