在配制香菇菌袋栽培料时添加0.2%(质量分数)增菇激活酶,研究增菇激活酶对菌丝生长周期、菌丝胞外酶活力、栽培料理化指标、生物学效率及子实体营养成分的影响.实验结果表明,添加增菇激活酶组的菌丝满袋时间为70 d,比对照组提前15 d.在菌丝转色期,增菇激活酶组菌丝纤维素酶、蛋白酶和淀粉酶活力持续增加,且显著高于对照组.出菇后采收子实体,增菇激活酶组的生物学效率达96.75%,比对照组提高21.99%,子实体营养成分略高于对照组,无显著差异.
The influence of mushroom activating enzymes on mycelium growth cycle,mycelium extracellular enzyme activity,physical and chemical indicators of cultivation material,biological efficiency and nutrient content of the Lentinus edodes fruiting body were studied during the fruiting body growth stage when 0.2% of mushroom activating enzymes were added in culture and the solid strains were inoclulated into the culture.The results showed that the time of mycelium growing full of the bags was 70 days, being shortened by 15 days compared with control.The cellulase、protease and amylase activity of mycelium in the mushroom activating enzymes group increased continuously during the colour changing period and the enzyme activities were significantly higher than that of control.After fruiting, the L. edodes fruiting body were collected and analyzed.The biological efficiency of the mushroom activating enzymes group achieved 96.75%,21.99% higher than that of control.The nutrient content was slightly higher than that of control,however,there was no significant difference between the two groups.
[1] KIRK P, MINTER D S. Dictionary of the fungi[M].10th deition. Oxon:CABI Europe-UK,2008.
[2] 余昌霞,汪虹,赵妍,等.两个香菇菌株的挥发性物质比较[J].食用菌学报,2017,24(4):65-70.DOI:10.16488/j.cnki.1005-9873.2017.04.013.
[3] 陈万超,杨焱,于海龙,等.七种干香菇主要营养成分与可溶性糖对比及电子舌分析[J].食用菌学报,2015,22(1):61-67.DOI:10.16488/j.cnki.1005-9873.2015.01.012.
[4] 魏雅冬,王广慧,李贺,等.香菇液体发酵条件的优化[J].山西农业科学,2016,44(4):506-508.DOI:10.3969/j.issn.1002-2481.2016.04.21.
[5] 刘敏,齐悦歌,申小燕,等.柿木屑基质下香菇培养基的优化[J].河北大学学报(自然科学版),2016,36(2):180-183.DOI:10.3969/j.issn.1000-1565.2016.02.012.
[6] ZHOU L D, ZHANG Q H, ZHANG Y, et al. The shiitake mushroom-derived immuno-stimulant lentinan protects against murine malaria blood-stage infection by evoking adaptive immune-responses[J].International Immunopharmacology,2009,9(4):455-462. DOI:10.1016/j.intimp.2009.01.010.
[7] ZHANG L N, LI X L, XU X J, et al. Correlation between antitumor activity,molecular weight, and conformation of lentinan[J].Carbohydrate Research,2005,340(8):1515-1521.DOI:10.1016/j.carres.2005.02.032.
[8] 王谦,刘利群,闫蕾蕾,等.香菇的深层发酵工艺应用研究[J].河北大学学报(自然科学版),2004,24(2):172-174.
[9] 王静之.激活剂对白色金针菇生长过程的影响研究[D].保定:河北大学,2016.
[10] 饲料添加剂纤维素酶活力的测定分光光度法NY/T912-2004[S].
[11] 饲料添加剂酸性、中性蛋白酶活力的测定GB/T28715-2012[S].
[12] 王林风,程远超.硝酸乙醇法测定纤维素含量[J].化学研究,2011,22(5):52-57.DOI:10.14002/j.hxya.2011.04.014.
[13] 食用菌中蛋白含量测定GB/T15673—2009[S].
