为了有效利用荆芥资源,采用菌丝生长速率法研究了荆芥醇提物对4种葡萄果实病原真菌的抑制作用.结果表明,当醇提物质量浓度为5 mg/mL时,对白腐病菌(Coniothyrium diplodiella)、炭疽病菌(Glomerella cingulate)、灰霉病菌(Botrytis cinerea)和炭黑曲霉病菌(Aspergillus carbonarius)的抑制率分别为94.57%、70.23%、71.15%和26.06%,对多菌灵高抗炭疽病菌菌株FJND-9、FJND-40和GZSD-89的抑制率分别为64.10%、68.73%和72.94%,对多菌灵高抗灰霉病菌菌株HBLF-10、GXZY-4和GXZY-7的抑制率分别为57.47%、66.83%和64.90%.本研究为葡萄病害非化学农药防控提供了依据.
For utilization of the resources of Nepeta cataria, the bioassay of ethanol extract against 4 pathogenic fungi isolated from grapes were carried out by mycelial growth rate method. The results showed that when the concentration of extract was 5.0 mg/mL, the inhibition rate against Coniothyrium diplodiella, Glomerella cingulate, Botrytis cinerea and Aspergillus carbonarius was 94.57%, 70.23%, 71.15% and 26.06%, respectively. The inhibition rate against carbendazim high resistant strains FJND-9, FJND-40 and GZSD-89 of G. cingulate was 64.10%, 68.73% and 72.94%,and the inhibition rate against carbendazim high resistant strains HBLF-10, GXZY-4 and GXZY-7 of B. cinerea was 57.47%, 66.83% and 64.90%, respectively, when ethanol extract was at same concentration. This study provides a basis for the prevention and control of grape diseases using non chemical pesticides.
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