将十六烷基三甲基溴化铵(CTAB)修饰前后的灭活烟曲霉菌丝体用于对阴离子偶氮染料刚果红的脱色,结果表明改性前烟曲霉菌丝体(AFB)和改性后的烟曲霉菌丝体(MAFB)均可有效去除染料溶液中刚果红.整个吸附过程的动力学过程符合伪二阶动力学模型,吸附数据符合Freundlich等温线模型,表明为多层吸附.采用不同的化学反应封闭烟曲霉的氨基、羧基以及羟基,结果表明烟曲霉氨基是吸附刚果红(CR)的主要基团,而羟基和羧基则起着一定的作用.烟曲霉菌丝对刚果红的脱色机理主要是零电荷点以下pH时,生物吸附剂的氨基、羟基和羧基发生质子化,带正电荷生物吸附剂与带负电荷CR通过静电吸引而被吸附,其次,吸附剂上的氨基、羟基和羧基与刚果红之间形成氢键而进一步被吸附;而零电荷点以上pH时,则主要通过形成氢键进行吸附.
The inactivated Aspergillus fumigatus mycelium before and after CTAB modification was used to decolorize Congo red, an anionic azo dye. The results showed that both the A. fumigatus mycelium(AFB)and the modified A. fumigatus mycelium(MAFB)could effectively remove Congo red from dye solution. The kinetics of the overall adsorption process was well described by pseudo-second order kinetic model and the sorption data fit Freundlich isotherm model well, indicating a multilayer adsorption behavior. Chemical modification of the functional groups of A.fumigatus suggested the major involvement of amino groups of A.fumigatus in CR adsorption,while hydroxyl and carboxyl groups played some role. The decolorization mechanism of Congo Red by Aspergillus fumigatus mycelium is described. Below the pH point of zero charge, the amino, hydroxyl and carboxyl groups of the biosorbent are protonated. Therefore,the positive charged biosorbent and the negative charge CR are bound to each other by electrostatic attraction. At the same time, the amino, hydroxyl and carboxyl groups on the biosorbent form hydrogen bonds with Congo Red. While above the pH point of zero charge, the main driving force is the hydrogen- DOI:10.3969/j.issn.1000-1565.2019.06.008改性烟曲霉对刚果红的去除动力学、等温线及机制金显春1,宋嘉宁2(1.河南农业大学 理学院,河南 郑州 450002;2、河南农业大学 林学院,河南 郑州 450002)摘 要:将十六烷基三甲基溴化铵(CTAB)修饰前后的灭活烟曲霉菌丝体用于对阴离子偶氮染料刚果红的脱色,结果表明改性前烟曲霉菌丝体(AFB)和改性后的烟曲霉菌丝体(MAFB)均可有效去除染料溶液中刚果红.整个吸附过程的动力学过程符合伪二阶动力学模型,吸附数据符合Freundlich等温线模型,表明为多层吸附.采用不同的化学反应封闭烟曲霉的氨基、羧基以及羟基,结果表明烟曲霉氨基是吸附刚果红(CR)的主要基团,而羟基和羧基则起着一定的作用.烟曲霉菌丝对刚果红的脱色机理主要是零电荷点以下pH时,生物吸附剂的氨基、羟基和羧基发生质子化,带正电荷生物吸附剂与带负电荷CR通过静电吸引而被吸附,其次,吸附剂上的氨基、羟基和羧基与刚果红之间形成氢键而进一步被吸附;而零电荷点以上pH时,则主要通过形成氢键进行吸附.关键词:烟曲霉;生物吸附;动力学;机制;等温线中图分类号:S435.72 文献标志码:A 文章编号:1000-1565(2019)06-0611-08Kinetics, isotherms and mechanisms of Congo red removal by modified Aspergillus fumigatusJIN Xianchun1,SONG Jianing2(1.School of Sciences, Henan Agricultural University, Zhengzhou 450002,China; 2.College of Forestry, Henan Agricultural University, Zhengzhou 450002,China)Abstract:The inactivated Aspergillus fumigatus mycelium before and after CTAB modification was used to decolorize Congo red, an anionic azo dye. The results showed that both the A. fumigatus mycelium(AFB)and the modified A. fumigatus mycelium(MAFB)could effectively remove Congo red from dye solution. The kinetics of the overall adsorption process was well described by pseudo-second order kinetic model and the sorption data fit Freundlich isotherm model well, indicating a multilayer adsorption behavior. Chemical modification of the functional groups of A.fumigatus suggested the major involvement of amino groups of A.fumigatus in CR adsorption,while hydroxyl and carboxyl groups played some role. The decolorization mechanism of Congo Red by Aspergillus fumigatus mycelium is described. Below the pH point of zero charge, the amino, hydroxyl and carboxyl groups of the biosorbent are protonated. Therefore,the positive charged biosorbent and the negative charge CR are bound to each other by electrostatic attraction. At the same time, the amino, hydroxyl and carboxyl groups on the biosorbent form hydrogen bonds with Congo Red. While above the pH point of zero charge, the main driving force is the hydrogen- 收稿日期:2018-09-16 基金项目:河南省高校重点科研项目(15A150051) 第一作者:金显春(1972—),女,湖南郴州人,河南农业大学副教授,博士,主要从事环境污染的微生物治理研究.E-mail:xchjin@163.com第6期金显春:改性烟曲霉对刚果红的去除动力学、等温线及机制bond formed between biosorbents and adsorbate.
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