为了建立白术Atractylodes macrocephala Koidz.通过胚轴、胚根外植体直接分化不定芽的高效离体再生体系,研究了外植体类型、植物生长调节剂种类及质量浓度对不定芽直接分化的影响.研究结果表明,以白术无菌苗的胚轴、胚根为外植体,在分化培养基上可直接诱导不定芽分化,其中胚轴不定芽分化率最高可达91.18%,每个外植体产生芽的数量最高可达13个;诱导不定芽分化的最佳培养基为MS(Murashige and Skoog)+ TDZ(thidiazuron)1.5 mg/L+ NAA(naphthylacetic acid)0.2 mg/L.将分化的不定芽培养于附加IBA(indol 3-butiric acid)0.5 mg/L的1/2 MS培养基中诱导生根,生根率达66.66%以上.本研究为药用植物白术的工厂化育苗和脱病毒以及利用植物基因工程技术进行品质改良提供了有效的再生途径.
For establishing the high efficiency regeneration system in vitro of Atractylodes macrocephala Koidz.,the effects of explant types and concentrations of plant growth regulators on the direct shoots regeneration for A.macrocephala were examined.The results indicated that shoots were directly induced from radicle and epicotyl explants without callus induction.The appropriate medium for shoot regeneration was MS + TDZ 1.5 mg/L + NAA 0.2 mg/L.With this regeneration system,91.18% of epicotyl explants produced an average of 13 elongated shoots per initial explant inoculated.The rooting efficiency was up to 66.66% on the 1/2 MS + IBA 0.5 mg/L.The regeneration system for A.macrocephala provided reliable technical support for plant genetic engineering quality improvement,industrial seeding,and virus-free of A.macrocephala.
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