以华北八宝Hylotelephium tatarinowii(Maxim.)H. Ohba愈伤组织为材料,采用不同质量分数的聚乙二醇(PEG)6 000进行胁迫处理,分别测定愈伤组织中过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性变化,通过非变性聚丙烯凝胶电泳分析POD、SOD和酯酶(EST)的同工酶表达特征.研究结果表明,在PEG 6 000质量分数为0.5%~5.0%时,随着质量分数的增加和胁迫时间的延长,POD、SOD和CAT的活性逐渐增强,且愈伤组织生长良好;当PEG 6 000质量分数为8.0%时,随着胁迫时间的延长,POD、SOD和CAT酶活性急剧下降.
Callus of Hylotelephium tatarinowii(Maxim.)H. Ohba was treated by different concentrations of polyethylene glycol(PEG 6 000)for stress treatment in the study. The activity of peroxidase(POD), superoxide dismutase(SOD)and catalase(CAT)in callus was measured, and isozyme expressions of POD, SOD and esterase(EST)were analyzed by non-denatured polypropylene gel electrophoresis. The activity of POD, SOD and CAT increased gradually with the increase of concentration and the prolongation of stress time when the concentration of PEG 6 000 was 0.5%—5.0%, and the growth state of callus was normal. When the concentration of PEG 6 000 was 8%, the activity of POD, SOD and CAT decreased sharply with the prolongation of stress time.
[1] 中科院中国植物志编委会. 中国植物志 第六十卷 第一分册[M]. 北京: 科学出版社, 1987.
[2] 黄德娟, 黄德超. 我国名贵香草植物及应用价值研究[J]. 北方园艺, 2007(3): 80-82. DOI:10.3969/j.issn.1001-0009.2007.03.031.
[3] 石进朝, 解有利, 迟全勃. 北京景天科野生植物引种栽培试验研究[J]. 北京农业职业学院学报, 2005, 19(3): 21-24. DOI:10.3969/j.issn.1671-7252.2005.03.005.
[4] 那冬晨, 王文斗, 杨丽静, 等. 水分胁迫对华北景天叶片结构和叶绿素含量的影响[J]. 安徽农业科学, 2011, 39(26): 15902-15903. DOI:10.3969/j.issn.0517-6611.2011.26.025.
[5] 那冬晨, 任彩琴, 柴婷婷, 等. 华北景天叶片有机渗透调节物质对水分胁迫的响应[J]. 北方园艺, 2011(17): 96-98.
[6] 张璐, 王俊丽, 王珏, 等. 华北八宝组织培养与再生体系的建立[J]. 河北大学学报(自然科学版), 2013, 33(6): 648-653. DOI:10.3969/j.issn.1000-1565.2013.06.017.
[7] 马林喜. 华北八宝再生过程中同工酶的研究[D]. 北京: 中央民族大学, 2015.
[8] SONG L L, DING W, ZHAO M G, et al. Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed[J]. Plant Science, 2006, 171(4): 449-458. DOI:10.1016/j.plantsci.2006.05.002.
[9] PORCEL R, BAREA J M, RUIZ-LOZANO J M. Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence[J]. New Phytologist, 2003, 157(1): 135-143. DOI:10.1046/j.1469-8137.2003.00658.x.
[10] DEMIRAL T, TURKAN I. Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment?[J]. Journal of Plant Physiology, 2004, 161(10): 1089-1100. DOI:10.1016/j.jplph.2004.03.009.
[11] 蔡小东, 崔航. PEG 6 000模拟干旱胁迫对柑橘愈伤组织抗氧化酶活性的影响[J]. 长江大学学报(自然科学版), 2013, 10(23): 14-17. DOI:10.16772/j.cnki.1673-1409.2013.23.011.
[12] 褚妍. PEG 6 000预处理对水分胁迫下水稻抗氧化酶同工酶及其表达的影响[D]. 沈阳: 沈阳师范大学, 2011.
