为了阐明大豆Glycine max(L.)Merr.胼胝质合酶在大豆抵御大豆花叶病毒(Soybean mosaic virus,SMV)侵染过程中的关键作用,采用生物信息学方法在大豆中鉴定出24个胼胝质合酶,并挑选出受一氧化氮(NO)影响且参与SMV抗性调控的大豆胼胝质合酶Glyma.08G308200(GmGSL7c).使用RT-qPCR验证了清除NO的大豆植株中基因GmGSL7c的表达情况,并借助基于烟草脆裂病毒(TRV)介导的基因沉默(VIGS)技术沉默GmGSL7c基因.结果发现,GmGSL7c的沉默影响了叶片中SMV诱导的胼胝质积累,在沉默植株未被接种的上位叶中可检测到SMV外壳蛋白基因SMV-CP并观察到发病症状,表明GmGSL7c基因的沉默抑制了大豆对SMV的抗性,为进一步研究大豆与SMV间互作的抗病信号通路奠定了基础.
This work centered on the key role of soybean callose synthase in the process of soybean mosaic virus infection. Twenty four callose synthase were identified in soybean by bioinformatics in this experiment. Glyma.08G308200(GmGSL7c)was selected for RT-qPCR to verify that the transcriptome data was consistent with the actual expression trend.TRV-VIGS followed by callose fluorescence observation found that silencing GmGSL7c affected the synthesis of callose in the leaves, and the SMV-CP gene could be detected in the upper leaves of the silenced plants that were not inoculated. In addition, disease symptoms of SMV were observed in the GmGSL7c silenced plants, indicating that the silencing of GmGSL7c promotes the transportation of SMV in soybeans. This study lays the foundation for further research on the soybean-SMV interaction signal pathway for disease resistance.
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