为有效利用藏橐吾Ligularia rumicifolia(Drumm.)S.W.Liu资源,以其子叶为外植体进行离体培养研究.结果发现,诱导愈伤组织的适宜培养基为MS+TDZ 1.0 mg/L+NAA 1.0 mg/L,诱导愈伤组织产生不定芽的适宜培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,诱导率为69.56%.诱导子叶产生不定芽的适宜培养基为MS+6-BA 3.0 mg/L+NAA 0.8 mg/L,诱导率为72.33%.不定芽在1/2 MS+IAA 0.5 mg/L的培养基中生根率为70.00%.
For utilization of Ligularia rumicifolia(Drumm.)S. W. Liu, tissue culture in vitro was tested using cotyledon as explants. The results showed that the MS medium containing TDZ 1.0 mg/L and NAA 1.0 mg/L was suitable for callus induction. The maximum induction rate of adventitious shoots from callus was 69.56% when NAA(0.5 mg/L)and 6-BA(2.0 mg/L)were added in the MS medium. The MS medium containing 6-BA 3.0 mg/L and NAA 0.8 mg/L gave the better response to adventitious shoots induction from cotyledon explants, and the induction rate reached up to 72.33%. About 70.00 % of the adventitious shoots rooted on 1/2 MS containing 0.5 mg/L IAA.
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